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DOI | 10.1186/s13059-019-1680-9 |
CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors | |
Butt, Haroon1; Eid, Ayman1; Momin, Afaque A.2; Bazin, Jeremie3; Crespi, Martin3; Arold, Stefan T.2; Mahfouz, Magdy M.1 | |
发表日期 | 2019 |
ISSN | 1474-760X |
卷号 | 20 |
英文摘要 | Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B1 mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of resistance to splicing inhibitors. Studies of the structural basis of the splicing inhibitor binding to SGRs corroborate the resistance phenotype. This directed evolution platform can be used to interrogate and evolve the molecular functions of key biomolecules and to engineer crop traits for improved performance and adaptation under climate change conditions. |
WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
来源期刊 | GENOME BIOLOGY
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文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/96720 |
作者单位 | 1.KAUST, Lab Genome Engn & Synthet Biol, Thuwal, Saudi Arabia; 2.KAUST, Div Biol & Environm Sci & Engn, Computat Biosci Res Ctr, Thuwal, Saudi Arabia; 3.Univ Paris Saclay, Univ Paris Diderot, Univ Evry,Sorbonne Paris Cite, Univ Paris Sud,INRA,CNRS,Inst Plant Sci Paris Sac, Orsay, France |
推荐引用方式 GB/T 7714 | Butt, Haroon,Eid, Ayman,Momin, Afaque A.,et al. CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors[J],2019,20. |
APA | Butt, Haroon.,Eid, Ayman.,Momin, Afaque A..,Bazin, Jeremie.,Crespi, Martin.,...&Mahfouz, Magdy M..(2019).CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors.GENOME BIOLOGY,20. |
MLA | Butt, Haroon,et al."CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors".GENOME BIOLOGY 20(2019). |
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