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DOI10.7717/peerj.6150
Using the wax moth larva Galleria mellonella infection model to detect emerging bacterial pathogens
Hernandez, Rafael J.1,4; Hesse, Elze2; Dowling, Andrea J.2; Coyle, Nicola M.3; Feil, Edward J.3; Gaze, Will H.4; Vos, Michiel4
发表日期2019
ISSN2167-8359
卷号6
英文摘要

Climate change, changing farming practices, social and demographic changes and rising levels of antibiotic resistance are likely to lead to future increases in opportunistic bacterial infections that are more difficult to treat. Uncovering the prevalence and identity of pathogenic bacteria in the environment is key to assessing transmission risks. We describe the first use of the Wax moth larva Galleria mellonella, a well-established model for the mammalian innate immune system, to selectively enrich and characterize pathogens from coastal environments in the South West of the UK. Whole-genome sequencing of highly virulent isolates revealed amongst others a Proteus mirabilis strain carrying the Salmonella SGI1 genomic island not reported from the UK before and the recently described species Vibrio injenensis hitherto only reported from human patients in Korea. Our novel method has the power to detect bacterial pathogens in the environment that potentially pose a serious risk to public health.


WOS研究方向Science & Technology - Other Topics
来源期刊PEERJ
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/91945
作者单位1.SUNY Stony Brook, Stony Brook Sch Med, Dept Global Med Educ, Stony Brook, NY 11794 USA;
2.Univ Exeter, Dept Biosci, Penryn, England;
3.Univ Bath, Milner Ctr Evolut, Dept Biol & Biochem, Bath, Avon, England;
4.Univ Exeter, European Ctr Environm & Human Hlth, Penryn, England
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Hernandez, Rafael J.,Hesse, Elze,Dowling, Andrea J.,et al. Using the wax moth larva Galleria mellonella infection model to detect emerging bacterial pathogens[J],2019,6.
APA Hernandez, Rafael J..,Hesse, Elze.,Dowling, Andrea J..,Coyle, Nicola M..,Feil, Edward J..,...&Vos, Michiel.(2019).Using the wax moth larva Galleria mellonella infection model to detect emerging bacterial pathogens.PEERJ,6.
MLA Hernandez, Rafael J.,et al."Using the wax moth larva Galleria mellonella infection model to detect emerging bacterial pathogens".PEERJ 6(2019).
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