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DOI | 10.1016/j.jviromet.2016.02.003 |
Optimization and evaluation of a method to detect adenoviruses in river water | |
McMinn, Brian R.; Korajkic, Asja; Grimm, Ann C. | |
发表日期 | 2016-05-01 |
ISSN | 0166-0934 |
卷号 | 231页码:8-13 |
英文摘要 | Adenoviruses are often implicated in recreational water disease outbreaks but existing methods for their detection perform poorly within these matrices. In this study, small volume (100 mL) concentration was used to identify processes that promoted recovery of adenovirus from river water. Several alternative secondary concentration techniques were investigated and compared to the baseline method consisting of primary concentration via filtration, followed by celite mediated secondary concentration. The alternative secondary concentrations included multiple filter elutions, soaking the filter for 15 min prior to elution and concentration using pre-treated celite (river water, 1.5% and 3% milk) instead of a filter. Modifications of the viral nucleic acid extraction technique were also evaluated. Concentration using pre-treated celite and a modified extraction technique (10 min boil and a 1 h ProK incubation at 37 degrees C) recovered significantly higher levels of adenovirus (P=0.001) than other methods tested. This optimized method increased recovery of spiked adenovirus (57 +/- 27%) compared to baseline method performance (4 3%) indicating that use of pre-treated celite as opposed to filtration significantly improves recovery. Application of the optimized concentration method to larger volume (1 L) of river water resulted in similar recoveries (42 +/- 19%) underlying the utility of this method to detect adenovirus from environmental samples. (C) 2016 Published by Elsevier B.V. |
英文关键词 | Adenovirus;River water;Small volume;Improved detection |
语种 | 英语 |
WOS记录号 | WOS:000374200400002 |
来源期刊 | JOURNAL OF VIROLOGICAL METHODS
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/62575 |
作者单位 | US EPA, 26 West Martin Luther King Dr, Cincinnati, OH 45268 USA |
推荐引用方式 GB/T 7714 | McMinn, Brian R.,Korajkic, Asja,Grimm, Ann C.. Optimization and evaluation of a method to detect adenoviruses in river water[J]. 美国环保署,2016,231:8-13. |
APA | McMinn, Brian R.,Korajkic, Asja,&Grimm, Ann C..(2016).Optimization and evaluation of a method to detect adenoviruses in river water.JOURNAL OF VIROLOGICAL METHODS,231,8-13. |
MLA | McMinn, Brian R.,et al."Optimization and evaluation of a method to detect adenoviruses in river water".JOURNAL OF VIROLOGICAL METHODS 231(2016):8-13. |
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