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DOI | 10.1021/acs.analchem.6b03730 |
Cobalamin Concentrations in Fetal Liver Show Gender Differences: A Result from Using a High-Pressure Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method | |
Bosle, Janine1; Goetz, Sven1; Raab, Andrea1; Krupp, Eva M.1; Schecke, Kirk G.2; Lorrb, Enzo3; Meharg, Andrew A.4; Fowler, Paul A.5; Feldmann, Jorg1 | |
发表日期 | 2016-12-20 |
ISSN | 0003-2700 |
卷号 | 88期号:24页码:12419-12426 |
英文摘要 | Maternal diet and lifestyle choices may affect placental transfer of cobalamin (Cbl) to the fetus. Fetal liver concentration of Cbl reflects nutritional status with regards to vitamin B12, but at these low concentration current Cbl measurement methods lack robustness. An analytical method based on enzymatic extraction with subsequent reversed-phase high -pressure liquid chromatography (RP-HPLC) separation and parallel ICPMS and electrospray ionization (ESI)-Orbitrap-MS to determine specifically Cbl species in liver samples of only 10-50 mg was developed using 14 pig livers. Subsequently 55 human fetal livers were analyzed. HPLC ICPMS analysis for cobalt (Co) and Cbl gave detection limits of 0.18 ng/g and 0.88 ng/g d.m. in liver samples, respectively, with a recovery of >95%. Total Co (Cot) concentration did not reflect the amount of Cbl or vitamin B12 in the liver. Cbl bound Co contributes only 45 +/- 15% to Cot. XRF mapping and mu XANES analysis confirmed the occurrence of non-Cbl cobalt in pig liver hot spots indicating particular Co. No correlations of total cobalt nor Cbl with fetal weight or weeks of gestation were found for the human fetal livers. Although no gender difference could be identified for total Co concentration, female livers were significantly higher in Cbl concentration (24.1 +/- 7.8 ng/g) than those from male fetuses (19.8 +/- 7.1 ng/g) (p = 0.04). This HPLC ICPMS method was able to quantify total Co-t and Cbl in fetus liver, and it was sensitive and precise enough to identify this gender difference. |
语种 | 英语 |
WOS记录号 | WOS:000390621000062 |
来源期刊 | ANALYTICAL CHEMISTRY
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/60107 |
作者单位 | 1.Univ Aberdeen, Dept Chem, TESLA Trace Element Speciat Lab, Meston Walk, Aberdeen AB24 3UE, Scotland; 2.US EPA, Natl Risk Management Res Lab, Land Remediat & Pollut Control Div, 5995 Ctr Hill Ave, Cincinnati, OH 45224 USA; 3.Univ South Australia, Future Ind Inst, Bldg X,Mawson Lakes Campus, Mawson Lakes, SA 5095, Australia; 4.Queens Univ Belfast, Inst Global Food Secur, Malone Rd, Belfast BT9 5BN, Antrim, North Ireland; 5.Univ Aberdeen, Sch Med Med Sci & Nutr, Inst Med Sci, Aberdeen AB25 2ZD, Scotland |
推荐引用方式 GB/T 7714 | Bosle, Janine,Goetz, Sven,Raab, Andrea,et al. Cobalamin Concentrations in Fetal Liver Show Gender Differences: A Result from Using a High-Pressure Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method[J]. 美国环保署,2016,88(24):12419-12426. |
APA | Bosle, Janine.,Goetz, Sven.,Raab, Andrea.,Krupp, Eva M..,Schecke, Kirk G..,...&Feldmann, Jorg.(2016).Cobalamin Concentrations in Fetal Liver Show Gender Differences: A Result from Using a High-Pressure Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method.ANALYTICAL CHEMISTRY,88(24),12419-12426. |
MLA | Bosle, Janine,et al."Cobalamin Concentrations in Fetal Liver Show Gender Differences: A Result from Using a High-Pressure Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method".ANALYTICAL CHEMISTRY 88.24(2016):12419-12426. |
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