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| DOI | 10.1289/ehp.1206301 |
| A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function | |
| Allard, Patrick1,2,3; Kleinstreuer, Nicole C.4; Knudsen, Thomas B.4; Colaiacovo, Monica P.1 | |
| 发表日期 | 2013-06-01 |
| ISSN | 0091-6765 |
| 卷号 | 121期号:6页码:717-724 |
| 英文摘要 | BACKGROUND: Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals. OBJECTIVES: We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure. METHODS: We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database. RESULTS: The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germ-line integrity by monitoring germ-line apoptosis and meiotic progression. CONCLUSIONS: This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germ-line function. |
| 英文关键词 | aneuploidy;C. elegans;chromosome segregation;germline;pesticides |
| 语种 | 英语 |
| WOS记录号 | WOS:000323709400022 |
| 来源期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES
 |
| 来源机构 | 美国环保署 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/59790 |
| 作者单位 | 1.Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA; 2.Univ Calif Los Angeles, Dept Environm Hlth Sci, Los Angeles, CA USA; 3.Univ Calif Los Angeles, Inst Soc & Genet, Los Angeles, CA USA; 4.US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA |
| 推荐引用方式 GB/T 7714 | Allard, Patrick,Kleinstreuer, Nicole C.,Knudsen, Thomas B.,et al. A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function[J]. 美国环保署,2013,121(6):717-724. |
| APA | Allard, Patrick,Kleinstreuer, Nicole C.,Knudsen, Thomas B.,&Colaiacovo, Monica P..(2013).A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function.ENVIRONMENTAL HEALTH PERSPECTIVES,121(6),717-724. |
| MLA | Allard, Patrick,et al."A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function".ENVIRONMENTAL HEALTH PERSPECTIVES 121.6(2013):717-724. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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