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DOI | 10.1016/j.mimet.2013.10.016 |
Development and validation of an open source O-2-sensitive gel for physiological profiling of soil microbial communities | |
McLamore, E. S.1; Garland, J. L.2; Mackowiak, C.3; Desaunay, A.1; Garland, N.1; Chaturvedi, P.1; Taguchi, M.1; Dreaden, K.1; Catechis, John4; Ullman, J. L.1 | |
发表日期 | 2014 |
ISSN | 0167-7012 |
卷号 | 96页码:62-67 |
英文摘要 | Community level physiological profiling is a simple, high-throughput technique for assessing microbial community physiology. Initial methods relying on redox-dye based detection of respiration were subject to strong enrichment bias, but subsequent development of a microtiter assay using an oxygen-quenched dye reduced this bias and improved the versatility of the approach. Commercial production of the oxygen microplates recently stopped, which led to the present effort to develop and validate a system using a luminophore dye (platinum tetrakis pentafluorophenyl) immobilized at the bottom of wells within a 96 well microtiter plate. The technique was used to analyze three well-characterized Florida soils: oak saw palmetto scrub, coastal mixed hardwood, and soil from an agricultural field used to grow corn silage. Substrate induced respiration was monitored by measuring respiration rates in soils under basal conditions and comparing to soils supplemented with nitrogen and various carbon sources (mannose, casein, asparagine, coumaric acid). All data was compared to a previously available commercial assay. There were no significant differences in the maximum peak intensity or the time to peak response for all soils tested (p < 0.001, alpha = 0.05). The experimental assay plates can be reused on soils up to four times (based on a deviation of less than 5%), where the commercial assay should not be reused. The results indicate that the new oxygen-based bioassay is a cost effective, open source tool for functional profiling of microbial communities. (C) 2013 Elsevier B.V. All tights reserved. |
英文关键词 | Physiological profiling;Oxygen quenched dye;Substrate induced respiration;Assay |
语种 | 英语 |
WOS记录号 | WOS:000329771000011 |
来源期刊 | JOURNAL OF MICROBIOLOGICAL METHODS
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/59411 |
作者单位 | 1.Univ Florida, Gainesville, FL USA; 2.US EPA, Microbiol & Chem Exposure Assessment Res Div, Cincinnati, OH 45268 USA; 3.Univ Florida, Dept Soil & Water Sci, Gainesville, FL USA; 4.Kennedy Space Ctr, Space Life Sci Lab, Kennedy Space Ctr, FL USA |
推荐引用方式 GB/T 7714 | McLamore, E. S.,Garland, J. L.,Mackowiak, C.,et al. Development and validation of an open source O-2-sensitive gel for physiological profiling of soil microbial communities[J]. 美国环保署,2014,96:62-67. |
APA | McLamore, E. S..,Garland, J. L..,Mackowiak, C..,Desaunay, A..,Garland, N..,...&Ullman, J. L..(2014).Development and validation of an open source O-2-sensitive gel for physiological profiling of soil microbial communities.JOURNAL OF MICROBIOLOGICAL METHODS,96,62-67. |
MLA | McLamore, E. S.,et al."Development and validation of an open source O-2-sensitive gel for physiological profiling of soil microbial communities".JOURNAL OF MICROBIOLOGICAL METHODS 96(2014):62-67. |
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