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DOI | 10.1128/AEM.03529-15 |
Biofilms on Hospital Shower Hoses: Characterization and Implications for Nosocomial Infections | |
Soto-Giron, Maria J.1,2; Rodriguez-R, Luis M.1,2; Luo, Chengwei2,3; Elk, Michael4; Ryu, Hodon5; Hoelle, Jill5; Domingo, Jorge W. Santo5; Konstantinidis, Konstantinos T.1,2,3 | |
发表日期 | 2016-05-01 |
ISSN | 0099-2240 |
卷号 | 82期号:9页码:2872-2883 |
英文摘要 | Although the source of drinking water (DW) used in hospitals is commonly disinfected, biofilms forming on water pipelines are a refuge for bacteria, including possible pathogens that survive different disinfection strategies. These biofilm communities are only beginning to be explored by culture-independent techniques that circumvent the limitations of conventional monitoring efforts. Hence, theories regarding the frequency of opportunistic pathogens in DW biofilms and how biofilm members withstand high doses of disinfectants and/or chlorine residuals in the water supply remain speculative. The aim of this study was to characterize the composition of microbial communities growing on five hospital shower hoses using both 16S rRNA gene sequencing of bacterial isolates and whole-genome shotgun metagenome sequencing. The resulting data revealed a Mycobacterium-like population, closely related to Mycobacterium rhodesiae and Mycobacterium tusciae, to be the predominant taxon in all five samples, and its nearly complete draft genome sequence was recovered. In contrast, the fraction recovered by culture was mostly affiliated with Proteobacteria, including members of the genera Sphingomonas, Blastomonas, and Porphyrobacter. The biofilm community harbored genes related to disinfectant tolerance (2.34% of the total annotated proteins) and a lower abundance of virulence determinants related to colonization and evasion of the host immune system. Additionally, genes potentially conferring resistance to beta-lactam, aminoglycoside, amphenicol, and quinolone antibiotics were detected. Collectively, our results underscore the need to understand the microbiome of DW biofilms using metagenomic approaches. This information might lead to more robust management practices that minimize the risks associated with exposure to opportunistic pathogens in hospitals. |
语种 | 英语 |
WOS记录号 | WOS:000374640400030 |
来源期刊 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/58863 |
作者单位 | 1.Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA; 2.Georgia Inst Technol, Ctr Bioinformat & Computat Genom, Atlanta, GA 30332 USA; 3.Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA; 4.Pegasus Inc, Cincinnati, OH USA; 5.US EPA, Off Res & Dev, Cincinnati, OH 45268 USA |
推荐引用方式 GB/T 7714 | Soto-Giron, Maria J.,Rodriguez-R, Luis M.,Luo, Chengwei,et al. Biofilms on Hospital Shower Hoses: Characterization and Implications for Nosocomial Infections[J]. 美国环保署,2016,82(9):2872-2883. |
APA | Soto-Giron, Maria J..,Rodriguez-R, Luis M..,Luo, Chengwei.,Elk, Michael.,Ryu, Hodon.,...&Konstantinidis, Konstantinos T..(2016).Biofilms on Hospital Shower Hoses: Characterization and Implications for Nosocomial Infections.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,82(9),2872-2883. |
MLA | Soto-Giron, Maria J.,et al."Biofilms on Hospital Shower Hoses: Characterization and Implications for Nosocomial Infections".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.9(2016):2872-2883. |
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