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DOI | 10.3390/pathogens6040054 |
Opportunistic Pathogens and Microbial Communities and Their Associations with Sediment Physical Parameters in Drinking Water Storage Tank Sediments | |
Qin, Ke1; Struewing, Ian2; Domingo, Jorge Santo3; Lytle, Darren3; Lu, Jingrang3 | |
发表日期 | 2017-12-01 |
ISSN | 2076-0817 |
卷号 | 6期号:4 |
英文摘要 | The occurrence and densities of opportunistic pathogens (OPs), the microbial community structure, and their associations with sediment elements from eight water storage tanks in Ohio, West Virginia, and Texas were investigated. The elemental composition of sediments was measured through X-ray fluorescence (XRF) spectra. The occurrence and densities of OPs and amoeba hosts (i.e., Legionella spp. and L. pneumophila, Mycobacterium spp., P. aeruginosa, V. vermiformis, Acanthamoeba spp.) were determined using genus- or species-specific qPCR assays. Microbial community analysis was performed using next generation sequencing on the Illumina Miseq platform. Mycobacterium spp. were most frequently detected in the sediments and water samples (88% and 88%), followed by Legionella spp. (50% and 50%), Acanthamoeba spp. (63% and 13%), V. vermiformis (50% and 25%), and P. aeruginosa (0 and 50%) by qPCR method. Comamonadaceae (22.8%), Sphingomonadaceae (10.3%), and Oxalobacteraceae (10.1%) were the most dominant families by sequencing method. Microbial communities in water samples were mostly separated with those in sediment samples, suggesting differences of communities between two matrices even in the same location. There were associations of OPs with microbial communities. Both OPs and microbial community structures were positively associated with some elements (Al and K) in sediments mainly from pipe material corrosions. Opportunistic pathogens presented in both water and sediments, and the latter could act as a reservoir of microbial contamination. There appears to be an association between potential opportunistic pathogens and microbial community structures. These microbial communities may be influenced by constituents within storage tank sediments. The results imply that compositions of microbial community and elements may influence and indicate microbial water quality and pipeline corrosion, and that these constituents may be important for optimal storage tank management within a distribution system. |
英文关键词 | Legionella;opportunistic pathogen;storage tank sediment;microbial community;element;corrosion |
语种 | 英语 |
WOS记录号 | WOS:000417347700010 |
来源期刊 | PATHOGENS
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/58291 |
作者单位 | 1.US EPA, Off Res & Dev, ORISE, Cincinnati, OH 45268 USA; 2.Pegasus Serv Inc, Cincinnati, OH 45268 USA; 3.US EPA, Off Res & Dev, Cincinnati, OH 45268 USA |
推荐引用方式 GB/T 7714 | Qin, Ke,Struewing, Ian,Domingo, Jorge Santo,et al. Opportunistic Pathogens and Microbial Communities and Their Associations with Sediment Physical Parameters in Drinking Water Storage Tank Sediments[J]. 美国环保署,2017,6(4). |
APA | Qin, Ke,Struewing, Ian,Domingo, Jorge Santo,Lytle, Darren,&Lu, Jingrang.(2017).Opportunistic Pathogens and Microbial Communities and Their Associations with Sediment Physical Parameters in Drinking Water Storage Tank Sediments.PATHOGENS,6(4). |
MLA | Qin, Ke,et al."Opportunistic Pathogens and Microbial Communities and Their Associations with Sediment Physical Parameters in Drinking Water Storage Tank Sediments".PATHOGENS 6.4(2017). |
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