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DOI10.1016/j.jviromet.2013.05.014
Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater
Brinkman, Nichole E.1,2; Haffler, Tyler D.1; Cashdollar, Jennifer L.1; Rhodes, Eric R.1
发表日期2013-10-01
ISSN0166-0934
卷号193期号:1页码:140-146
英文摘要

Enteroviruses, noroviruses and adenoviruses are among the most common viruses infecting humans worldwide. These viruses are shed in the feces of infected individuals and can accumulate in wastewater, making wastewater a source of a potentially diverse group of enteric viruses. In this study, two procedures were evaluated to concentrate noroviruses, adenoviruses and enteroviruses from primary effluent of wastewater. In the first procedure, indigenous enteroviruses, noroviruses and adenoviruses were concentrated using celite (diatomaceous earth) followed by centrifugation through a 30K MWCO filter and nucleic acid extraction. The second procedure used celite concentration followed by nucleic acid extraction only. Virus quantities were measured using qPCR. A second set of primary effluent samples were seeded with Coxsackievirus A7, Coxsackievirus B1, poliovirus 1 or enterovirus 70 before concentration and processed through both procedures for recovery evaluation of enterovirus species representatives. The pairing of the single step extraction procedure with the celite concentration process resulted in 47-98% recovery of examined viruses, while the celite concentration process plus additional centrifugal concentration before nucleic acid extraction showed reduced recovery (14-47%). The celite concentration process followed by a large volume nucleic acid extraction technique proved to be an effective procedure for recovering these important human pathogens from wastewater. Published by Elsevier B.V.


英文关键词Norovirus;Adenovirus;Enterovirus;Wastewater;Celite
语种英语
WOS记录号WOS:000323362100022
来源期刊JOURNAL OF VIROLOGICAL METHODS
来源机构美国环保署
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/57285
作者单位1.US EPA, Biohazard Assessment Res Branch, Microbiol & Chem Exposure Assessment Res Div, Natl Exposure Res Lab,Off Res & Dev, Cincinnati, OH 45268 USA;
2.Univ Cincinnati, McMicken Coll Arts & Sci, Dept Biol Sci, Cincinnati, OH 45221 USA
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GB/T 7714
Brinkman, Nichole E.,Haffler, Tyler D.,Cashdollar, Jennifer L.,et al. Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater[J]. 美国环保署,2013,193(1):140-146.
APA Brinkman, Nichole E.,Haffler, Tyler D.,Cashdollar, Jennifer L.,&Rhodes, Eric R..(2013).Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater.JOURNAL OF VIROLOGICAL METHODS,193(1),140-146.
MLA Brinkman, Nichole E.,et al."Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater".JOURNAL OF VIROLOGICAL METHODS 193.1(2013):140-146.
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