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DOI | 10.1016/j.jviromet.2013.05.014 |
Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater | |
Brinkman, Nichole E.1,2; Haffler, Tyler D.1; Cashdollar, Jennifer L.1; Rhodes, Eric R.1 | |
发表日期 | 2013-10-01 |
ISSN | 0166-0934 |
卷号 | 193期号:1页码:140-146 |
英文摘要 | Enteroviruses, noroviruses and adenoviruses are among the most common viruses infecting humans worldwide. These viruses are shed in the feces of infected individuals and can accumulate in wastewater, making wastewater a source of a potentially diverse group of enteric viruses. In this study, two procedures were evaluated to concentrate noroviruses, adenoviruses and enteroviruses from primary effluent of wastewater. In the first procedure, indigenous enteroviruses, noroviruses and adenoviruses were concentrated using celite (diatomaceous earth) followed by centrifugation through a 30K MWCO filter and nucleic acid extraction. The second procedure used celite concentration followed by nucleic acid extraction only. Virus quantities were measured using qPCR. A second set of primary effluent samples were seeded with Coxsackievirus A7, Coxsackievirus B1, poliovirus 1 or enterovirus 70 before concentration and processed through both procedures for recovery evaluation of enterovirus species representatives. The pairing of the single step extraction procedure with the celite concentration process resulted in 47-98% recovery of examined viruses, while the celite concentration process plus additional centrifugal concentration before nucleic acid extraction showed reduced recovery (14-47%). The celite concentration process followed by a large volume nucleic acid extraction technique proved to be an effective procedure for recovering these important human pathogens from wastewater. Published by Elsevier B.V. |
英文关键词 | Norovirus;Adenovirus;Enterovirus;Wastewater;Celite |
语种 | 英语 |
WOS记录号 | WOS:000323362100022 |
来源期刊 | JOURNAL OF VIROLOGICAL METHODS
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/57285 |
作者单位 | 1.US EPA, Biohazard Assessment Res Branch, Microbiol & Chem Exposure Assessment Res Div, Natl Exposure Res Lab,Off Res & Dev, Cincinnati, OH 45268 USA; 2.Univ Cincinnati, McMicken Coll Arts & Sci, Dept Biol Sci, Cincinnati, OH 45221 USA |
推荐引用方式 GB/T 7714 | Brinkman, Nichole E.,Haffler, Tyler D.,Cashdollar, Jennifer L.,et al. Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater[J]. 美国环保署,2013,193(1):140-146. |
APA | Brinkman, Nichole E.,Haffler, Tyler D.,Cashdollar, Jennifer L.,&Rhodes, Eric R..(2013).Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater.JOURNAL OF VIROLOGICAL METHODS,193(1),140-146. |
MLA | Brinkman, Nichole E.,et al."Evaluation of methods using celite to concentrate norovirus, adenovirus and enterovirus from wastewater".JOURNAL OF VIROLOGICAL METHODS 193.1(2013):140-146. |
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