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DOI | 10.1111/lam.12725 |
A novel broth medium for enhanced growth of Francisella tularensis | |
Morris, B. J.1; Buse, H. Y.2; Adcock, N. J.2; Rice, E. W.2 | |
发表日期 | 2017-06-01 |
ISSN | 0266-8254 |
卷号 | 64期号:6页码:394-400 |
英文摘要 | Francisella tularensis is a fastidious organism that requires a lengthy incubation time in liquid growth media for detection. The objective of this study was to develop a medium formulation using readily available supplements that enhanced early growth of F. tularensis. Francisella tularensis live vaccine strain was used to evaluate the growth responses for each of the media formulations tested. Growth in brain heart infusion broth supplemented with 2% Vitox, 10% Fildes and 1% histidine (BVFH) resulted in a significant increase in growth after 8h incubation compared to other media formulations tested (P<0001). Virulent strains of F. tularensis grown in BVFH medium demonstrated similar enhanced early growth. Cell densities of 39-52 log(10) CFU per ml were obtained after 24h of growth in BVFH from a 1-2cellml(-1) starting inoculum of the virulent Type A Schu4 strain, indicating the suitability of this medium in rapidly amplifying low starting titres of F. tularensis. Collectively, these results indicate that the novel formulation of the BVFH medium was capable of producing enhanced growth response for F. tularensis. Significance and Impact of the StudyThe need for rapid cultivation of Francisella tularensis is essential for detection and monitoring during natural outbreak events or intentional bioterrorism attacks. The addition of selected supplements into the base medium BHI (BVFH) developed in this study enhanced growth of F. tularensis Type A1, A2 and B strains compared to BHI alone. Growth of these organisms in BVFH will allow for improved response time should a natural or intentional contamination event occur. |
英文关键词 | antibiotics;detection;environmental;rapid methods;virulence |
语种 | 英语 |
WOS记录号 | WOS:000401148600001 |
来源期刊 | LETTERS IN APPLIED MICROBIOLOGY |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/57121 |
作者单位 | 1.Pegasus Tech Serv Inc, Cincinnati, OH USA; 2.US EPA, Natl Homeland Secur Res Ctr, 26 W Martin Luther King Dr NG 16, Cincinnati, OH 45268 USA |
推荐引用方式 GB/T 7714 | Morris, B. J.,Buse, H. Y.,Adcock, N. J.,et al. A novel broth medium for enhanced growth of Francisella tularensis[J]. 美国环保署,2017,64(6):394-400. |
APA | Morris, B. J.,Buse, H. Y.,Adcock, N. J.,&Rice, E. W..(2017).A novel broth medium for enhanced growth of Francisella tularensis.LETTERS IN APPLIED MICROBIOLOGY,64(6),394-400. |
MLA | Morris, B. J.,et al."A novel broth medium for enhanced growth of Francisella tularensis".LETTERS IN APPLIED MICROBIOLOGY 64.6(2017):394-400. |
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