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Two common laboratory extraction techniques were evaluated for routine use with the micro-colorimetric lipid determination method developed by Van Handel (1985) [2] and recently validated for small samples by Inouye and Lotufo (2006) [1]. With the accelerated solvent extraction method using chloroform: methanol solvent and the colorimetric lipid determination method, 28 of 30 samples had significant proportional bias (alpha=1%, determined using standard additions) and 1 of 30 samples had significant constant bias (alpha= 1%, determined using Youden Blank measurements). With sonic extraction, 0 of 6 samples had significant proportional bias (alpha= 1%) and 1 of 6 samples had significant constant bias (alpha=1%). These demonstrate that the accelerated solvent extraction method with chloroform:methanol solvent system creates an interference with the colorimetric assay method, and without accounting for the bias in the analysis, inaccurate measurements would be obtained. Published by Elsevier B.V.