气候变化领域集成服务门户

Bacterial community in the water of Lake Puma Yumco, a freshwater alpine lake on the Tibetan Plateau was studied for the first time using an integrated approach including water chemistry and culture-dependent and -independent methods. Microbial abundance in the lake water was from 1.59 105 to 3.37 105 cells mL- 1, and bacteria were evenly distributed in the lake due to its uniform water physical-chemistry. Obtained isolates were affiliated with -, - and -Proteobacteria, Actinobacteria and Bacteroidetes, among which -Proteobacteria isolates were dominant. Retrieved bacterial 16S rRNA gene clones fell into -, - and -Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Planctomycetes, Verrucomicrobia and Chloroflexi. Bacterial diversity in Lake Puma Yumco was higher than that in other Tibetan lakes at levels of both phyla and clusters. A half of total clones in Lake Puma Yumco were members of the widespread typical freshwater bacterial clusters. The low temperature of Lake Puma Yumco also made it possess unique freshwater bacteria members.