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DOI	10.1128/AEM.71.8.4619-4627.2005
	16S rRNA sequences and differences in bacteria isolated from the Muztag Ata glacier at increasing depths
	Xiang, SR; Yao, TD; An, LZ; Xu, BL; Wang, JX
发表日期	2005
ISSN	0099-2240
EISSN	1098-5336
起始页码	4619
结束页码	4627
卷号	71 期号:8 页码:4619-4627
英文摘要	Small subunit 16S rRNA sequences, growth temperatures, and phylogenetic relationships have been established for 129 bacterial isolates recovered under aerobic growth conditions from different regions of a 22-m ice core from the Muztag Ata Mountain glacier on the Pamirs Plateau (China). Only 11% were psychrophiles (grew at 2 degrees C or -2 degrees C up to similar to 20 degrees C), although the majority (82%) were psychrotolerant (grew at 2 degrees C or -2 degrees C up to 37 degrees C). The majority of the isolates had 16S rRNA sequences similar to previously determined sequences, ranging from 85% to 100% identical to database sequences. Based on their 16S rRNA sequences, 42.6% of the isolates were high-G+C (HGC) gram-positive bacteria, 23.3% were gamma-Proteobacteria, 14.7% were alpha-Proteobacteria, 14.7% were Flavobacteria, and 4.7% were low-G+C (LGC) gram-positive bacteria. There were clear differences in the depth distribution, with Proteobacteria, HGC/Cytophaga-Flavobacterium-Bacteroides (CFB), Proteobacteria, LGC/CFB/HGC, Cryobacterium psychrophilum, HGC/CFB, Proteobacteria/HGC/CFB, and HGC/CFB being the predominant isolates from ice that originated from 2.7 to 3.8, 6.2, 7.5, 8.3, 9.0, 9.7, 12.5, and 15.3 m below the surface, respectively. This layered distribution of bacterial isolates presumably reflects both differences in bacteria inhabiting the glacier's surface, differences in bacteria deposited serendipitously on the glacier's surface by wind and snowfall, and nutrient availability within the ice.
关键词	MALAN ICE CORE SP-NOV.PSYCHROPHILIC BACTERIUM VIABLE BACTERIA LAKE VOSTOK SNOW ALGAE DIVERSITY COMMUNITY TEMPERATURE RECOVERY
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000231165500059
来源期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/451
推荐引用方式 GB/T 7714	Xiang, SR,Yao, TD,An, LZ,et al. 16S rRNA sequences and differences in bacteria isolated from the Muztag Ata glacier at increasing depths[J]. 中国科学院青藏高原研究所,2005,71(8):4619-4627.
APA	Xiang, SR,Yao, TD,An, LZ,Xu, BL,&Wang, JX.(2005).16S rRNA sequences and differences in bacteria isolated from the Muztag Ata glacier at increasing depths.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,71(8),4619-4627.
MLA	Xiang, SR,et al."16S rRNA sequences and differences in bacteria isolated from the Muztag Ata glacier at increasing depths".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 71.8(2005):4619-4627.