气候变化领域集成服务门户(CCPortal): Plasma and milk metabolomics revealed changes in amino acid metabolism in Holstein dairy cows under heat stress

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DOI	10.1016/j.animal.2023.101049
	Plasma and milk metabolomics revealed changes in amino acid metabolism in Holstein dairy cows under heat stress
	Jorge-Smeding, E.; Leung, Y. H.; Ruiz-Gonzalez, A.; Xu, W.; Astessiano, A. L.; Trujillo, A. I.; Rico, D. E.; Kenez, A.
发表日期	2024
ISSN	1751-7311
EISSN	1751-732X
起始页码	18
结束页码	2
卷号	18 期号:2
英文摘要	Our understanding of metabolic alterations triggered by heat stress is incomplete, which limits the designing of nutritional strategies to mitigate negative productive and health effects. Thus, this study aimed to explore the metabolic responses of heat-stressed dairy cows to dietary supplementation with vitamin D-3/Ca and vitamin E/Se. Twelve multiparous Holstein cows were enrolled in a split-plot Latin square design with two distinct vitamin E/Se supplementation levels, either at a low (ESe-, n = 6, 11.1 IU/kg vitamin E and 0.55 mg/kg Se) or a high dose (ESe+, n = 6 223 IU/kg vitamin E and 1.8 mg/ kg Se) as the main plot. Treatment subplots, arranged in a replicated 3 x 3 Latin square design, comprised heat challenge (Temperature Humidity Index, THI: 72.0-82.0) supplemented with different levels of vitamin D3/Ca: either low (HS/DCa-, 1 012 IU/kg and 0.73%, respectively) or high (HS/DCa+, 3 764 IU/kg and 0.97%, respectively), and a pair-fed control group in thermoneutrality (THI = 61.0-64.0) receiving the low dose of vitamin D-3/Ca (TN). The liquid chromatography-mass spectrometry-based metabolome profile was determined in blood plasma and milk sampled at the beginning (day 0) and end (day 14) of each experimental period. The results were analyzed for the effect of (1) TN vs. HS/ESe-/DCa-, and (2) the vitamin E/Se and vitamin D-3/Ca supplementation. No group or group x day effects were detected in the plasma metabolome (false discovery rate, FDR > 0.05), except for triglyceride 52:2 being higher (FDR = 0.03) on day 0 than 14. Taurine, creatinine and butyryl-carnitine showed group x day interactions in the milk metabolome (FDR < 0.05) as creatinine (+22%) and butyryl-carnitine (+190%) were increased (P < 0.01) on day 14, and taurine was decreased (-65%, P < 0.01) on day 14 in the heat stress (HS) cows, compared with day 0. Most compounds were unaffected by vitamin E/Se or vitamin D3/Ca supplementation level or their interaction (FDR > 0.05) in plasma and milk, except for milk alanine which was lower (-69%, FDR = 0.03) in the E/Se+ groups, compared with E/Se-. Our results indicated that HS triggered more prominent changes in the milk than in the plasma metabolome, with consistent results in milk suggesting increased muscle catabolism, as reflected by increased creatinine, alanine and citrulline levels. Supplementing with high levels of vitamin E/Se or vitamin D3/Ca or their combination did not appear to affect the metabolic remodeling triggered by HS. (c) 2023 The Author(s). Published by Elsevier B.V. on behalf of The Animal Consortium.
英文关键词	Climate change; Protein catabolism; Ruminant nutrition; Systems biology; Thermal regulation
语种	英语
WOS研究方向	Agriculture ; Veterinary Sciences
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS记录号	WOS:001156553100001
来源期刊	ANIMAL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/306489
作者单位	City University of Hong Kong; Universidad de la Republica, Uruguay; Laval University; KU Leuven
推荐引用方式 GB/T 7714	Jorge-Smeding, E.,Leung, Y. H.,Ruiz-Gonzalez, A.,et al. Plasma and milk metabolomics revealed changes in amino acid metabolism in Holstein dairy cows under heat stress[J],2024,18(2).
APA	Jorge-Smeding, E..,Leung, Y. H..,Ruiz-Gonzalez, A..,Xu, W..,Astessiano, A. L..,...&Kenez, A..(2024).Plasma and milk metabolomics revealed changes in amino acid metabolism in Holstein dairy cows under heat stress.ANIMAL,18(2).
MLA	Jorge-Smeding, E.,et al."Plasma and milk metabolomics revealed changes in amino acid metabolism in Holstein dairy cows under heat stress".ANIMAL 18.2(2024).