气候变化领域集成服务门户(CCPortal): Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka

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DOI	10.1007/s11686-024-00829-8
	Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka
	Amarasingha, Sakunika; Anupama, N. M. T.; Rajakaruna, Rupika S.; Rajapakse, R. P. V. J.; Perera, Piyumali K.
发表日期	2024
ISSN	1230-2821
EISSN	1896-1851
英文摘要	Purpose Soil-transmitted nematodes (STNs) are widespread in tropical and subtropical regions, particularly where the communities are socio-economically challenged. We investigated the effect of soil temperature on the prevalence and intensity of STN infection in free-roaming dogs. Methods Fresh faecal samples collected from free-roaming dogs in Digana and Pussellawa town areas in the Kandy District, Sri Lanka, were microscopically analysed for canine STNs. Soil temperature was measured at each sampling site. Highly prevalent canine hookworm Ancylostoma, was further studied using PCR and sequencing, followed by phylogenetic analysis. Results The soil temperature ranged between 28 and 31 degrees C (mean = 29.79 degrees C) and 18-21 degrees C (mean = 19.52 degrees C) in Digana and Pussellawa, respectively, showing a significant difference in the two sites (Students t-test t = 1.68, p < 0.0001). Of the total 44 dogs sampled, 41 (93.2%) were positive for STNs. During microscopic analysis, five nematodes: Ancylostoma spp., Capillaria sp., Strongyloides sp., Toxocara canis, and Trichuris sp., were identified. Ancylostoma species (93.2%) were the most prevalent, followed by Strongyloides sp. (22.7%) and Toxocara canis (15.9%). Infection prevalence of Strongyloides sp. was higher in Digana (40.9%) compared to that in Pussellawa (4.5%; Chi-square test, chi(2) = 8.28, p = 0.004) and also the infection intensity from Digana (EPG = 8.02 +/- 20.2) compared to that from Pussellawa (0.45 +/- 2.1; Mann Whitney U test, p = 0.006). Amplicons (ITS1-5.8S-ITS2) of the expected size for A. caninum, and A. tubaeforme were produced. An A. caninum sequence reported here (OQ101719) illustrated the highest similarity of 99.2% to one of the local sequences (MZ707153) upon pairwise comparison. Conclusion Digana, with a higher soil temperature than Pussellawa, had a significantly higher prevalence and infection intensity, particularly Strongyloides sp. This study also signifies the first molecular identification of hookworm species A. tubaeforme in Sri Lanka.
英文关键词	Dog; Soil-transmitted nematodes; Sri Lanka; Soil temperature; Zoonotic; Ancylostoma tubaeforme; Hookworm
语种	英语
WOS研究方向	Parasitology ; Veterinary Sciences ; Zoology
WOS类目	Parasitology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:001190126800001
来源期刊	ACTA PARASITOLOGICA
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/304658
作者单位	University of Peradeniya; University of Peradeniya
推荐引用方式 GB/T 7714	Amarasingha, Sakunika,Anupama, N. M. T.,Rajakaruna, Rupika S.,et al. Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka[J],2024.
APA	Amarasingha, Sakunika,Anupama, N. M. T.,Rajakaruna, Rupika S.,Rajapakse, R. P. V. J.,&Perera, Piyumali K..(2024).Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka.ACTA PARASITOLOGICA.
MLA	Amarasingha, Sakunika,et al."Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka".ACTA PARASITOLOGICA (2024).