气候变化领域集成服务门户(CCPortal): Unlocking the potential of rice for thermotolerance using simple sequence repeat (SSR) marker-based assessment of genetic variability and population structure

CCPortal

DOI	10.1007/s10722-024-02022-2
	Unlocking the potential of rice for thermotolerance using simple sequence repeat (SSR) marker-based assessment of genetic variability and population structure
	Seelam, Ravi Teja; Puram, Venkata Ramana Rao; Nallamothu, Veronica; Injeti, Sudhir Kumar; Madhunapantula, Vani Praveena
发表日期	2024
ISSN	0925-9864
EISSN	1573-5109
英文摘要	Climate change due to global warming is causing an increase in heat stress in rice (Oryza sativa L.). Heat stress reduces the yield of rice, which is one of the major staple crop for half of the world's population. Therefore, there is a dire need to breed heat-tolerant rice cultivars. In any crop improvement program, the presence of genetic diversity and evaluation of the structural association are crucial for developing the breeding strategy. This study was conducted to examine the genetic variability and population structure of 49 rice genotypes, including both released rice cultivars and advanced breeding lines using 75 simple sequence repeat (SSR markers) linked to thermotolerance located across the 12 rice chromosomes. There were a total of 113 alleles, with their number ranging from 2 to 4 and having a mean of 2.31 alleles per locus. The average values for polymorphic information content (PIC), genetic diversity and major allele frequency were 0.36, 0.35 and 0.71 respectively. Among the markers evaluated, RM4108 exhibited highest PIC value (0.65) and gene diversity (0.64), thereby indicating that it is highly polymorphic marker and an ideal choice for characterizing thermotolerance rice cultivars. Both genetic distance-based clustering analysis and population structure revealed that the 49 genotypes formed three significant clusters. Cluster 1 comprised a mixture of 7 released cultivars and 12 breeding lines, followed by cluster 2, which included 11 released cultivars and 8 breeding lines. Cluster 3 consisted a total of 11 released cultivars. Furthermore, the principal coordinate analysis demonstrated cumulative genetic variations of 15.60%, 14.17%, 8.03% and 7.02% among the mixed populations. Most of the released cultivars were placed in coordinate 1, while breeding lines were found in coordinate 3. The analysis of Maruteru rice cultivars revealed a diverse genetic composition and the obtained information will be valuable for establishing an association panel and selecting suitable parents for future rice development programs.
英文关键词	Thermotolerance; Genetic variability; Population structure; SSR markers; Maruteru rice cultivars
语种	英语
WOS研究方向	Agriculture ; Plant Sciences
WOS类目	Agronomy ; Plant Sciences
WOS记录号	WOS:001229325900001
来源期刊	GENETIC RESOURCES AND CROP EVOLUTION
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/304270
作者单位	Acharya N. G. Ranga Agricultural University; University System of Georgia; University of Georgia; Acharya N. G. Ranga Agricultural University; Acharya N. G. Ranga Agricultural University; Acharya N. G. Ranga Agricultural University
推荐引用方式 GB/T 7714	Seelam, Ravi Teja,Puram, Venkata Ramana Rao,Nallamothu, Veronica,et al. Unlocking the potential of rice for thermotolerance using simple sequence repeat (SSR) marker-based assessment of genetic variability and population structure[J],2024.
APA	Seelam, Ravi Teja,Puram, Venkata Ramana Rao,Nallamothu, Veronica,Injeti, Sudhir Kumar,&Madhunapantula, Vani Praveena.(2024).Unlocking the potential of rice for thermotolerance using simple sequence repeat (SSR) marker-based assessment of genetic variability and population structure.GENETIC RESOURCES AND CROP EVOLUTION.
MLA	Seelam, Ravi Teja,et al."Unlocking the potential of rice for thermotolerance using simple sequence repeat (SSR) marker-based assessment of genetic variability and population structure".GENETIC RESOURCES AND CROP EVOLUTION (2024).