气候变化领域集成服务门户(CCPortal): Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses

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DOI	10.1186/s13071-023-06109-0
	Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses
	Terradas, Gerard; Manzano-Alvarez, Jaime; Vanalli, Chiara; Werling, Kristine; Cattadori, Isabella M.; Rasgon, Jason L.
发表日期	2024
ISSN	1756-3305
起始页码	17
结束页码	1
卷号	17 期号:1
英文摘要	Background Increasing global temperatures and unpredictable climatic extremes have contributed to the spread of vector-borne diseases. The mosquito Aedes aegypti is the main vector of multiple arboviruses that negatively impact human health, mostly in low socioeconomic areas of the world. Co-circulation and co-infection of these viruses in humans have been increasingly reported; however, how vectors contribute to this alarming trend remains unclear. Methods Here, we examine single and co-infection of Mayaro virus (D strain, Alphavirus) and dengue virus (serotype 2, Flavivirus) in Ae. aegypti adults and cell lines at two constant temperatures, moderate (27 degree celsius) and hot (32 degree celsius), to quantify vector competence and the effect of temperature on infection, dissemination and transmission, including on the degree of interaction between the two viruses. Results Both viruses were primarily affected by temperature but there was a partial interaction with co-infection. Dengue virus quickly replicates in adult mosquitoes with a tendency for higher titers in co-infected mosquitoes at both temperatures, and mosquito mortality was more severe at higher temperatures in all conditions. For dengue, and to a lesser extent Mayaro, vector competence and vectorial capacity were higher at hotter temperature in co- vs. single infections and was more evident at earlier time points (7 vs. 14 days post infection) for Mayaro. The temperature-dependent phenotype was confirmed in vitro by faster cellular infection and initial replication at higher temperatures for dengue but not for Mayaro virus. Conclusions Our study suggests that contrasting kinetics of the two viruses could be related to their intrinsic thermal requirements, where alphaviruses thrive better at lower temperatures compared to flaviviruses. However, more studies are necessary to clarify the role of co-infection at different temperature regimes, including under more natural temperature settings.
英文关键词	Climate change; Arbovirus; Vector-borne disease; Temperature; Co-infection; Emerging pathogens
语种	英语
WOS研究方向	Parasitology ; Tropical Medicine
WOS类目	Parasitology ; Tropical Medicine
WOS记录号	WOS:001166971700004
来源期刊	PARASITES & VECTORS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/302848
作者单位	Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park
推荐引用方式 GB/T 7714	Terradas, Gerard,Manzano-Alvarez, Jaime,Vanalli, Chiara,et al. Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses[J],2024,17(1).
APA	Terradas, Gerard,Manzano-Alvarez, Jaime,Vanalli, Chiara,Werling, Kristine,Cattadori, Isabella M.,&Rasgon, Jason L..(2024).Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses.PARASITES & VECTORS,17(1).
MLA	Terradas, Gerard,et al."Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses".PARASITES & VECTORS 17.1(2024).