气候变化领域集成服务门户(CCPortal): Identification and functional analysis of a chromosome 2D fragment harboring TaFPF1 gene with the potential for yield improvement using a late heading wheat mutant

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DOI	10.1007/s00122-024-04593-1
	Identification and functional analysis of a chromosome 2D fragment harboring TaFPF1 gene with the potential for yield improvement using a late heading wheat mutant
	Wu, Lifen; Li, Guangrong; Li, Danping; Dong, Chunhao; Zhang, Xueying; Zhang, Lichao; Yang, Zujun; Kong, Xiuying; Xia, Chuan; Chen, Jingtang; Liu, Xu
发表日期	2024
ISSN	0040-5752
EISSN	1432-2242
起始页码	137
结束页码	4
卷号	137 期号:4
英文摘要	In recent years, wheat production has faced formidable challenges driven by rapid population growth and climate change, emphasizing the importance of improving specific agronomic traits such as heading date, spike length, and grain size. To identify potential genes for improving these traits, we screened a wheat EMS mutant library and identified a mutant, designated m406, which exhibited a significantly delayed heading date compared to the wild-type. Intriguingly, the mutant also displayed significantly longer spike and larger grain size. Genetic analysis revealed that a single recessive gene was responsible for the delayed heading. Surprisingly, a large 46.58 Mb deletion at the terminal region of chromosome arm 2DS in the mutant was identified through fine mapping and fluorescence in situ hybridization. Thus, the phenotypes of the mutant m406 are controlled by a group of linked genes. This deletion encompassed 917 annotated high-confidence genes, including the previously studied wheat genes Ppd1 and TaDA1, which could affect heading date and grain size. Multiple genes in this region probably contribute to the phenotypes of m406. We further investigated the function of TaFPF1 using gene editing. TaFPF1 knockout mutants showed delayed heading and increased grain size. Moreover, we identified the direct upstream gene of TaFPF1 and investigated its relationship with other important flowering genes. Our study not only identified more genes affecting heading and grain development within this deleted region but also highlighted the potential of combining these genes for improvement of wheat traits.
语种	英语
WOS研究方向	Agriculture ; Plant Sciences ; Genetics & Heredity
WOS类目	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
WOS记录号	WOS:001197238600001
来源期刊	THEORETICAL AND APPLIED GENETICS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/301502
作者单位	Hebei Agricultural University; Chinese Academy of Agricultural Sciences; Institute of Crop Sciences, CAAS
推荐引用方式 GB/T 7714	Wu, Lifen,Li, Guangrong,Li, Danping,et al. Identification and functional analysis of a chromosome 2D fragment harboring TaFPF1 gene with the potential for yield improvement using a late heading wheat mutant[J],2024,137(4).
APA	Wu, Lifen.,Li, Guangrong.,Li, Danping.,Dong, Chunhao.,Zhang, Xueying.,...&Liu, Xu.(2024).Identification and functional analysis of a chromosome 2D fragment harboring TaFPF1 gene with the potential for yield improvement using a late heading wheat mutant.THEORETICAL AND APPLIED GENETICS,137(4).
MLA	Wu, Lifen,et al."Identification and functional analysis of a chromosome 2D fragment harboring TaFPF1 gene with the potential for yield improvement using a late heading wheat mutant".THEORETICAL AND APPLIED GENETICS 137.4(2024).