气候变化领域集成服务门户(CCPortal): Analysis of genetic diversity and population structure in some Egyptian Berseem (Trifolium alexandrinum) accessions based on ISSR, SCoT and SRAP markers

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DOI	10.1007/s10722-024-01940-5
	Analysis of genetic diversity and population structure in some Egyptian Berseem (Trifolium alexandrinum) accessions based on ISSR, SCoT and SRAP markers
	Mohamed, Hagar Mostafa; El-Assal, Salah El-Din Sayed Mohamed; El-Din, Abdel-Kader Youssef Gamal; El-Khishin, Dina Aziz
发表日期	2024
ISSN	0925-9864
EISSN	1573-5109
英文摘要	Egyptian clover (Trifolium alexandrinum) has received limited attention regarding its genetic diversity, rendering it susceptible to climate change. To develop resilient Berseem cultivars, exploring its genetic resources is vital for enhancing the diversity within its germplasm, thereby optimizing breeding programs. The examination of 24 Berseem accessions. Using three different molecular marker types; Inter-Simple Sequence Repeat (ISSR) (10 primers), Start Codon Targeted polymorphism (SCoT) (22 primers), and Sequence-Related Amplified Polymorphism (SRAP) (17 primer combinations) revealed a high genetic variability. ISSR markers had the highest polymorphism rate (82.2%) compared to SCoT (69.6%) and SRAP (65.2%). Unweighted Pair Group Method with Arithmetic Mean clustering analysis divided the studied accessions into two major groups, supported by STRUCTURE analysis, which also split samples into two subpopulations ( Delta K = 2). Additionally, this classification was verified by both principal component analysis and a heat map. The study underscores the importance of Berseem's genetic diversity in crop development and breeding, with ISSR markers offering valuable insights due to their high polymorphism. Despite the diverse geographic origins of the accessions, the lack of geographic clustering implies significant genetic mixing, possibly because of high outcrossing facilitated by insect pollination or self-incompatibility. The combined use of multiple markers deepens our understanding of Berseem's genetics by providing more detailed and comprehensive coverage of the clover genome, allowing for increased resolution, validation of results, and a holistic perspective on the genomic landscape, furthermore, identifying and recommending specific Berseem accessions (NGB-43724, NGB-19680, Gemeiza1, and Giza6) as prospective candidates for future breeding programs in Egypt.
英文关键词	Trifolium; Berseem; ISSR; SCoT; SRAP; Genetic diversity
语种	英语
WOS研究方向	Agriculture ; Plant Sciences
WOS类目	Agronomy ; Plant Sciences
WOS记录号	WOS:001190481400003
来源期刊	GENETIC RESOURCES AND CROP EVOLUTION
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/300184
作者单位	Egyptian Knowledge Bank (EKB); Agricultural Research Center - Egypt; Egyptian Knowledge Bank (EKB); Cairo University
推荐引用方式 GB/T 7714	Mohamed, Hagar Mostafa,El-Assal, Salah El-Din Sayed Mohamed,El-Din, Abdel-Kader Youssef Gamal,et al. Analysis of genetic diversity and population structure in some Egyptian Berseem (Trifolium alexandrinum) accessions based on ISSR, SCoT and SRAP markers[J],2024.
APA	Mohamed, Hagar Mostafa,El-Assal, Salah El-Din Sayed Mohamed,El-Din, Abdel-Kader Youssef Gamal,&El-Khishin, Dina Aziz.(2024).Analysis of genetic diversity and population structure in some Egyptian Berseem (Trifolium alexandrinum) accessions based on ISSR, SCoT and SRAP markers.GENETIC RESOURCES AND CROP EVOLUTION.
MLA	Mohamed, Hagar Mostafa,et al."Analysis of genetic diversity and population structure in some Egyptian Berseem (Trifolium alexandrinum) accessions based on ISSR, SCoT and SRAP markers".GENETIC RESOURCES AND CROP EVOLUTION (2024).