气候变化领域集成服务门户(CCPortal): Proteomic characterization of the different stages of seed germination in Cupressus gigantea

CCPortal

DOI	10.1017/S0960258524000126
	Proteomic characterization of the different stages of seed germination in Cupressus gigantea
	Li, Jianxin; Zhang, Subin; Lei, Pei; Eh, Tong-Ju; Zhang, Yu; Jin, Guangze; Dong, Kun; Xu, Qijiang; Luo, Qiuxiang; Meng, Fanjuan
发表日期	2024
ISSN	0960-2585
EISSN	1475-2735
英文摘要	Seed germination is a pivotal period of plant growth and development. This process can be divided into four major stages, swelling absorption, seed coat dehiscence, radicle emergence and radicle elongation. Cupressus gigantea, a tree native to Tibet, China, is characterized by its resistance to stresses such as cold, and drought, and has a high economic and ecological value. Nevertheless, given its unique geographic location, its seeds are difficult to germinate. Therefore, it is crucial to explore the mechanisms involved in seed germination in this species to improve the germination efficiency of its seeds, thereby protecting this high-quality resource. Here, our findings indicate that seed germination was enhanced when exposed to a 6-h/8-h light/dark photoperiod, coupled with a temperature of 20 degrees C. Furthermore, the application of exogenous GA(3) (1 mg/ml, about 2.9 mM) stimulated the germination of C. gigantea seeds. Subsequently, proteomics was used to detect changes in protein expression during the four stages of seed germination. We identified 34 differentially expressed proteins (DEPs), including 13 at the radicle pre-emergence stage, and 17 at the radicle elongation stage. These DEPs were classified into eight functional groups, cytoskeletal proteins, energy metabolism, membrane transport, stress response, molecular chaperones, amino acid metabolism, antioxidant system and ABA signalling pathway. Most of them were found to be closely associated with amino acid metabolism. Combined, these findings indicate that, along with temperature and light, exogenous GA(3) can increase the germination efficiency of C. gigantea seeds. Our study also offers insights into the changes in protein expression patterns in C. gigantea seeds during germination.
英文关键词	Cupressus gigantea; proteomics; seed germination; two-dimensional electrophoresis (2-DE)
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001217240200001
来源期刊	SEED SCIENCE RESEARCH
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/300059
作者单位	Jilin Agricultural University; Northeast Forestry University - China; Heilongjiang Academy of Agricultural Sciences; Northeast Forestry University - China; Northeast Forestry University - China; Northeast Forestry University - China; Youjiang Medical University for Nationalities
推荐引用方式 GB/T 7714	Li, Jianxin,Zhang, Subin,Lei, Pei,et al. Proteomic characterization of the different stages of seed germination in Cupressus gigantea[J],2024.
APA	Li, Jianxin.,Zhang, Subin.,Lei, Pei.,Eh, Tong-Ju.,Zhang, Yu.,...&Meng, Fanjuan.(2024).Proteomic characterization of the different stages of seed germination in Cupressus gigantea.SEED SCIENCE RESEARCH.
MLA	Li, Jianxin,et al."Proteomic characterization of the different stages of seed germination in Cupressus gigantea".SEED SCIENCE RESEARCH (2024).