气候变化领域集成服务门户(CCPortal): Overexpression of the plastidial pseudo-protease AtFtsHi3 enhances drought tolerance while sustaining plant growth

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DOI	10.1111/ppl.14370
	Overexpression of the plastidial pseudo-protease AtFtsHi3 enhances drought tolerance while sustaining plant growth
	Mishra, Laxmi S.; Cook, Sam D.; Kushwah, Sunita; Isaksson, Hanna; Straub, Isabella R.; Abele, Miriam; Mishra, Sanatkumar; Ludwig, Christina; Libby, Eric; Funk, Christiane
发表日期	2024
ISSN	0031-9317
EISSN	1399-3054
起始页码	176
结束页码	3
卷号	176 期号:3
英文摘要	With climate change, droughts are expected to be more frequent and severe, severely impacting plant biomass and quality. Here, we show that overexpressing the Arabidopsis gene AtFtsHi3 (FtsHi3OE) enhances drought-tolerant phenotypes without compromising plant growth. AtFtsHi3 encodes a chloroplast envelope pseudo-protease; knock-down mutants (ftshi3-1) are found to be drought tolerant but exhibit stunted growth. Altered AtFtsHi3 expression therefore leads to drought tolerance, while only diminished expression of this gene leads to growth retardation. To understand the underlying mechanisms of the enhanced drought tolerance, we compared the proteomes of ftshi3-1 and pFtsHi3-FtsHi3OE (pFtsHi3-OE) to wild-type plants under well-watered and drought conditions. Drought-related processes like osmotic stress, water transport, and abscisic acid response were enriched in pFtsHi3-OE and ftshi3-1 mutants following their enhanced drought response compared to wild-type. The knock-down mutant ftshi3-1 showed an increased abundance of HSP90, HSP93, and TIC110 proteins, hinting at a potential downstream role of AtFtsHi3 in chloroplast pre-protein import. Mathematical modeling was performed to understand how variation in the transcript abundance of AtFtsHi3 can, on the one hand, lead to drought tolerance in both overexpression and knock-down lines, yet, on the other hand, affect plant growth so differently. The results led us to hypothesize that AtFtsHi3 may form complexes with at least two other protease subunits, either as homo- or heteromeric structures. Enriched amounts of AtFtsH7/9, AtFtsH11, AtFtsH12, and AtFtsHi4 in ftshi3-1 suggest a possible compensation mechanism for these proteases in the hexamer.
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001236091000001
来源期刊	PHYSIOLOGIA PLANTARUM
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/297949
作者单位	Umea University; Umea University; Umea University; Technical University of Munich; Swedish University of Agricultural Sciences; Umea University; Swedish University of Agricultural Sciences
推荐引用方式 GB/T 7714	Mishra, Laxmi S.,Cook, Sam D.,Kushwah, Sunita,et al. Overexpression of the plastidial pseudo-protease AtFtsHi3 enhances drought tolerance while sustaining plant growth[J],2024,176(3).
APA	Mishra, Laxmi S..,Cook, Sam D..,Kushwah, Sunita.,Isaksson, Hanna.,Straub, Isabella R..,...&Funk, Christiane.(2024).Overexpression of the plastidial pseudo-protease AtFtsHi3 enhances drought tolerance while sustaining plant growth.PHYSIOLOGIA PLANTARUM,176(3).
MLA	Mishra, Laxmi S.,et al."Overexpression of the plastidial pseudo-protease AtFtsHi3 enhances drought tolerance while sustaining plant growth".PHYSIOLOGIA PLANTARUM 176.3(2024).