气候变化领域集成服务门户(CCPortal): Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina

CCPortal

DOI	10.1007/s13237-024-00490-6
	Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina
	Najar, Mushtaq Ahmad; Gangophadhay, Gaurab
发表日期	2024
ISSN	0029-568X
EISSN	0976-7975
英文摘要	Sesame (Sesamum indicum L.) suffers significant production loss due to the opportunistic fungus Macrophomina phaseolina which causes charcoal rot. Climate change-induced increasing temperatures and irregular seasonal patterns create the propitious disease triangle necessary for the infestation of this destructive fungus. This demands an improved sesame genotype for enhanced charcoal rot tolerance, achieved through our novel inter-specific hybrid (S. indicum x S. mulayanum). To get a global overview of transcripts responsive to induced infection of M. phaseolina, RNA-sequencing of cultivated (S. indicum), wild (S. mulayanum) and inter-specific hybrid was performed. Several potential differentially expressed genes (DEGs) which could foster an understanding of the Mp-sesame-pathosystem, were subjected to fluorescence-based quantitative real-time PCR (RT-qPCR). Potential candidate genes were annotated with model plant Arabidopsis to find putative functions based on conserved sequence similarity. Bioinformatics analysis was performed to gain insights into cellular localization, isoelectric point and protein molecular weight of identified candidate genes. Our combined results of the disease susceptibility index, and expression analysis of candidate genes highlighted the role of cell wall-related genes, phenylpropanoid pathway, hydrolases and expression trends in inter-specific hybrid, suggested enhanced tolerance to charcoal rot. Our study findings would bolster the understanding of the defense mechanism of sesame in response to M. phaseolina.
英文关键词	Sesame; Macrophomina infection; Transcriptomic data; Defense genes; Validation
语种	英语
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:001205907000001
来源期刊	NUCLEUS-INDIA
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/297631
作者单位	Department of Science & Technology (India); Bose Institute
推荐引用方式 GB/T 7714	Najar, Mushtaq Ahmad,Gangophadhay, Gaurab. Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina[J],2024.
APA	Najar, Mushtaq Ahmad,&Gangophadhay, Gaurab.(2024).Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina.NUCLEUS-INDIA.
MLA	Najar, Mushtaq Ahmad,et al."Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina".NUCLEUS-INDIA (2024).