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| DOI | 10.1007/s11240-023-02636-x |
| Efficient genetic transformation of rice using Agrobacterium with a codon-optimized chromoprotein reporter gene (ChromoP) and introducing an optimized iPCR method for transgene integration site detection | |
| 发表日期 | 2024 |
| ISSN | 0167-6857 |
| EISSN | 1573-5044 |
| 起始页码 | 156 |
| 结束页码 | 1 |
| 卷号 | 156期号:1 |
| 英文摘要 | Rice (Oryza sativa L.) is a vital staple food for a significant portion of the global population. Meeting the demand and enhancing productivity in the face of climate change and population growth necessitate innovative strategies, including molecular modification, genetic engineering, and genome editing. This study presents a simplified gene transformation protocol for the rice cultivar Hashemi using 4 to 7-day-old calluses. The method, based on mature rice seeds and gene transformation to a 4 to 7-day-old callus, achieves a maximum 40% efficiency, circumventing complications associated with using immature. Notably, a novel reporter gene, ChromoP (a codon-optimized chromoprotein), is introduced for the first time to facilitate the optimization of gene transformation. The transgenic calli containing ChromoP exhibit a distinctive pinkish-purple coloration, enabling easy visual identification. Furthermore, the study introduces a simple, fast, and cost-effective protocol, named semi-universal inverse PCR (SUN-iPCR), for detecting individual events and determining the insertion site of a transgene. In conclusion, this study successfully outlines a gene transformation protocol for rice, covering the process until the detection of an independent transformant. Furthermore, the reporter gene and SUN-iPCR methodology utilized in this study hold promising potential for optimizing gene transformation in various other plant species. |
| 英文关键词 | Rice; Agrobacterium-mediated transformation; Chromoprotein reporter gene (ChromoP); SUN-iPCR; Individual events |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS类目 | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS记录号 | WOS:001119789500003 |
| 来源期刊 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/297152 |
| 作者单位 | Pohang University of Science & Technology (POSTECH); University of Kurdistan |
| 推荐引用方式 GB/T 7714 | . Efficient genetic transformation of rice using Agrobacterium with a codon-optimized chromoprotein reporter gene (ChromoP) and introducing an optimized iPCR method for transgene integration site detection[J],2024,156(1). |
| APA | (2024).Efficient genetic transformation of rice using Agrobacterium with a codon-optimized chromoprotein reporter gene (ChromoP) and introducing an optimized iPCR method for transgene integration site detection.PLANT CELL TISSUE AND ORGAN CULTURE,156(1). |
| MLA | "Efficient genetic transformation of rice using Agrobacterium with a codon-optimized chromoprotein reporter gene (ChromoP) and introducing an optimized iPCR method for transgene integration site detection".PLANT CELL TISSUE AND ORGAN CULTURE 156.1(2024). |
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