气候变化领域集成服务门户(CCPortal): Graphene quantum dots enhance the biological nitrogen fixation by up-regulation of cellular metabolism and electron transport

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DOI	10.1016/j.cej.2024.150694
	Graphene quantum dots enhance the biological nitrogen fixation by up-regulation of cellular metabolism and electron transport
	Ouyang, Bowei; Zhong, Qinmei; Ouyang, Peng; Yuan, Yue; Wu, Xian; Yang, Sheng-Tao
发表日期	2024
ISSN	1385-8947
EISSN	1873-3212
起始页码	487
卷号	487
英文摘要	Graphene quantum dots (GQDs) are useful nanomaterials of excellent water solubility, biocompatibility and optical stability for various applications. In this study, we achieved the nitrogen fixation activity enhancement of autogenous azotobacters (Azotobacter vinelandii) by GQDs and revealed its mechanism. GQDs (1-10 mg/L) accelerated the logarithmic growth phase of A. vinelandii, making bacteria enter the plateau earlier. Biocompatible GQDs did not cause cell death and oxidative damage of A. vinelandii. The expression levels of metabolism and nitrogen fixation related genes were significantly up-regulated by GQDs, thereby enhancing the activity of nitrogenase. The nitrogen fixation activity of A. vinelandii reached 471.7 % of the control upon the exposure to GQDs. The intracellular polyhydroxyalkanoate vesicles were consumed to provide energy for nitrogen fixation, which involved with a chain reaction of carbohydrate metabolism, lipid metabolism, and energy metabolism pathways accompanied by significant increases in electron transfer rate. The synergistic effect of GQDs on A. vinelandii further facilitated the augmentation of soil nitrogen content in Qinghai-Tibet Plateau. Our results provided an effective approach to enhance the biological nitrogen fixation by GQDs.
英文关键词	Graphene quantum dots; Nitrogen fixation activity; Nitrogenase; Gene regulation; Environmental bioeffects
语种	英语
WOS研究方向	Engineering
WOS类目	Engineering, Environmental ; Engineering, Chemical
WOS记录号	WOS:001217874600001
来源期刊	CHEMICAL ENGINEERING JOURNAL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/293842
作者单位	Southwest Minzu University; China University of Geosciences; Southwest Minzu University
推荐引用方式 GB/T 7714	Ouyang, Bowei,Zhong, Qinmei,Ouyang, Peng,et al. Graphene quantum dots enhance the biological nitrogen fixation by up-regulation of cellular metabolism and electron transport[J],2024,487.
APA	Ouyang, Bowei,Zhong, Qinmei,Ouyang, Peng,Yuan, Yue,Wu, Xian,&Yang, Sheng-Tao.(2024).Graphene quantum dots enhance the biological nitrogen fixation by up-regulation of cellular metabolism and electron transport.CHEMICAL ENGINEERING JOURNAL,487.
MLA	Ouyang, Bowei,et al."Graphene quantum dots enhance the biological nitrogen fixation by up-regulation of cellular metabolism and electron transport".CHEMICAL ENGINEERING JOURNAL 487(2024).