气候变化领域集成服务门户(CCPortal): Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds

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DOI	10.1111/pbi.14340
	Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds
	Trinh, Mai Duy Luu; Visintainer, Davide; Gunther, Jan; Osterberg, Jeppe Thulin; da Fonseca, Rute R.; Fondevilla, Sara; Moog, Max William; Luo, Guangbin; Norrevang, Anton F.; Crocoll, Christoph; Nielsen, Philip V.; Jacobsen, Sven-Erik; Wendt, Toni; Bak, Soren; Lopez-Marques, Rosa Laura; Palmgren, Michael
发表日期	2024
ISSN	1467-7644
EISSN	1467-7652
英文摘要	Climate change may result in a drier climate and increased salinization, threatening agricultural productivity worldwide. Quinoa (Chenopodium quinoa) produces highly nutritious seeds and tolerates abiotic stresses such as drought and high salinity, making it a promising future food source. However, the presence of antinutritional saponins in their seeds is an undesirable trait. We mapped genes controlling seed saponin content to a genomic region that includes TSARL1. We isolated desired genetic variation in this gene by producing a large mutant library of a commercial quinoa cultivar and screening the library for specific nucleotide substitutions using droplet digital PCR. We were able to rapidly isolate two independent tsarl1 mutants, which retained saponins in the leaves and roots for defence, but saponins were undetectable in the seed coat. We further could show that TSARL1 specifically controls seed saponin biosynthesis in the committed step after 2,3-oxidosqualene. Our work provides new important knowledge on the function of TSARL1 and represents a breakthrough for quinoa breeding.
英文关键词	Chenopodium quinoa; genome-wide association study; mutagenesis; quinoa; saponin biosynthesis; site-directed genotype screening
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号	WOS:001196332100001
来源期刊	PLANT BIOTECHNOLOGY JOURNAL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/293353
作者单位	University of Copenhagen; University of Copenhagen; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto de Agricultura Sostenible (IAS)
推荐引用方式 GB/T 7714	Trinh, Mai Duy Luu,Visintainer, Davide,Gunther, Jan,et al. Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds[J],2024.
APA	Trinh, Mai Duy Luu.,Visintainer, Davide.,Gunther, Jan.,Osterberg, Jeppe Thulin.,da Fonseca, Rute R..,...&Palmgren, Michael.(2024).Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds.PLANT BIOTECHNOLOGY JOURNAL.
MLA	Trinh, Mai Duy Luu,et al."Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds".PLANT BIOTECHNOLOGY JOURNAL (2024).