气候变化领域集成服务门户(CCPortal): RNAi-based genetically engineered rice resistant to black-streaked dwarf virus does not show adverse genetic effects: A multi-omics analysis

CCPortal

DOI	10.1002/ppp3.10477
	RNAi-based genetically engineered rice resistant to black-streaked dwarf virus does not show adverse genetic effects: A multi-omics analysis
	Huang, Chunmeng; Wang, Zhi; Zhu, Pengyu; Xu, Wenjie; Du, Zhixin; Qian, Yike; Fu, Wei
发表日期	2024
EISSN	2572-2611
起始页码	6
结束页码	3
卷号	6 期号:3
英文摘要	Societal Impact StatementAdvancements in science and technology enable us to address the challenges of the times: hunger, malnutrition, climate change, and dwindling natural resources. One of the most significant innovations in food and agriculture is the advent of genetic engineering, which may help us address the aforementioned challenges. Millions of people could benefit from the application of genetically engineered crops in agriculture and food. However, it is vital that biosafety assessments of unintended effects are carried out to identify any potential risks to humans, the environment, or society.SummaryThe study compared the extent of siRNA, transcriptomic and metabolomic changes in the leaves of four RNAi-based genetically engineered rice lines to evaluate whether RNAi-based genetically engineered rice that is resistant to black-streaked dwarf virus experiences changes not seen in the non-genetically engineered counterpart.siRNA, transcriptome, and metabolome profiling were conducted. siRNAs with different lengths and abundance were highly enriched in the transcript of rice; however, relative expression analysis of eight potential off-target genes revealed that there was no decrease in gene expression in the RNAi-based genetically engineered lines, meaning that no off-target phenomena were caused as a result of siRNA derived from invert repeat sequences of rice black-streaked dwarf virus in this study.This study identified some differentially expressed genes and differentially accumulated metabolites in the RNAi-based genetically engineered lines; however, they were not enriched in pathways detrimental for humans, animals and the environment. Only 30 differentially expressed genes were significantly enriched in the plant hormone signal transduction pathway via a combined analysis using two-way orthogonal partial least squares model. These genes belonging to salicylic acid, jasmonic acid, cytokinin, abscisic acid, and brassinosteroid pathway involved in pathways related to virus resistance or infection response were significantly changed.Taken together, there was no off-target in RNAi-based genetically engineered rice lines and may have a positive effect on virus resistance overall in this study. Advancements in science and technology enable us to address the challenges of the times: hunger, malnutrition, climate change, and dwindling natural resources. One of the most significant innovations in food and agriculture is the advent of genetic engineering, which may help us address the aforementioned challenges. Millions of people could benefit from the application of genetically engineered crops in agriculture and food. However, it is vital that biosafety assessments of unintended effects are carried out to identify any potential risks to humans, the environment, or society.image
英文关键词	combined analysis; metabolome; RNAi-based genetically engineered rice; siRNA; transcriptome; unintended effect
语种	英语
WOS研究方向	Biodiversity & Conservation ; Plant Sciences ; Environmental Sciences & Ecology
WOS类目	Biodiversity Conservation ; Plant Sciences ; Ecology
WOS记录号	WOS:001148334600001
来源期刊	PLANTS PEOPLE PLANET
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/293062
作者单位	Chinese Academy of Inspection & Quarantine; China Agricultural University; Jianghan University
推荐引用方式 GB/T 7714	Huang, Chunmeng,Wang, Zhi,Zhu, Pengyu,et al. RNAi-based genetically engineered rice resistant to black-streaked dwarf virus does not show adverse genetic effects: A multi-omics analysis[J],2024,6(3).
APA	Huang, Chunmeng.,Wang, Zhi.,Zhu, Pengyu.,Xu, Wenjie.,Du, Zhixin.,...&Fu, Wei.(2024).RNAi-based genetically engineered rice resistant to black-streaked dwarf virus does not show adverse genetic effects: A multi-omics analysis.PLANTS PEOPLE PLANET,6(3).
MLA	Huang, Chunmeng,et al."RNAi-based genetically engineered rice resistant to black-streaked dwarf virus does not show adverse genetic effects: A multi-omics analysis".PLANTS PEOPLE PLANET 6.3(2024).