气候变化领域集成服务门户(CCPortal): Human fascioliasis emergence in southern Asia: Complete nuclear rDNA spacer and mtDNA gene sequences prove Indian patient infection related to fluke hybridization in northeastern India and Bangladesh

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DOI	10.1016/j.onehlt.2024.100675
	Human fascioliasis emergence in southern Asia: Complete nuclear rDNA spacer and mtDNA gene sequences prove Indian patient infection related to fluke hybridization in northeastern India and Bangladesh
	Bargues, M. Dolores; Artigas, Patricio; Varghese, George M.; John, T. Jacob; Ajjampur, Sitara S. R.; Ahasan, Syed Ali; Chowdhury, Emdadul Haque; Gabrielli, Albis Francesco; Mas-Coma, Santiago
发表日期	2024
EISSN	2352-7714
起始页码	18
卷号	18
英文摘要	Fascioliasis is a snail-borne zoonotic disease with impact on the development of human subjects and communities. It is caused by two liver-infecting fasciolid trematode species, the globally-distributed Fasciola hepatica and the Africa/Asia-restricted but more pathogenic, larger F. gigantica. Fasciola gigantica is the cause of endemicity in livestock throughout the warm lowlands from Pakistan to southeastern Asia since old times. Human fascioliasis is emerging in this region at present, with an increase of patient reports. Complete sequences of rDNA ITS-1 and ITS-2 spacers and mtDNA nad1 and cox1 genes were obtained from fasciolid eggs found in the endoscopic bile aspirate from a patient of Arunachal Pradesh, northeastern India. Egg measurements, pronounced ITS heterozygosity, and pure F. gigantica mtDNA haplotypes demonstrate an infection by a recent F. gigantica-like hybrid. Sequence identities and similarities with the same DNA markers found in livestock from Bangladesh prove the human-infecting fasciolid to present identical ITSs and nad1 haplotypes and only one silent transversion in cox1 when compared to a widely-spread combined haplotype in animals. In northeastern India and Bangladesh, human fascioliasis emergence appears linked to increasing livestock prevalences due to: ruminant importation from other countries because of the increasing demand of rapidly growing human populations; numerous livestock movements, including transborder corridors, due to the uncontrolled small-scale household farming practices; and man-made introduction of F. hepatica with imported livestock into an area originally endemic for F. gigantica leading to frequent hybridization. Sequences, phylogenetic trees, and networks indicate that the origins of intermediate/hybrid fasciolids and factors underlying human infection risk differ in eastern and western South Asia. The emergence scenario in southern China and Vietnam resembles the aforementioned of northeastern India and Bangladesh, whereas in Pakistan it is linked to increasing monsoon rainfall within climate change combined with an impact of an extensive irrigation system. Past human-guided movements of pack animals along the western Grand Trunk Road and the eastern Tea-Horse Road explain the F. gigantica mtDNA results obtained. Physicians should be aware about these emerging scenarios, clinical pictures, diagnostic techniques and treatment. Government authorities must appropriately warn health professionals, ensure drug availability and improve livestock control.
英文关键词	Fasciola gigantica and F. hepatica; Human infection; Nuclear rDNA ITS-2 and ITS-1; Mitochondrial DNA nad1 and cox1; Encoded proteins; Complete Sequences; Heterozygotic sequence complexity; Crossbreeding hybrids; Livestock infection; Animal importation; Arunachal Pradesh; India; Bangladesh; Pakistan; China; Vietnam
语种	英语
WOS研究方向	Public, Environmental & Occupational Health ; Infectious Diseases
WOS类目	Public, Environmental & Occupational Health ; Infectious Diseases
WOS记录号	WOS:001173908700001
来源期刊	ONE HEALTH
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/293015
作者单位	University of Valencia; Instituto de Salud Carlos III; Christian Medical College & Hospital (CMCH) Vellore; Christian Medical College & Hospital (CMCH) Vellore; Christian Medical College & Hospital (CMCH) Vellore; Bangladesh Agricultural University (BAU); World Health Organization
推荐引用方式 GB/T 7714	Bargues, M. Dolores,Artigas, Patricio,Varghese, George M.,et al. Human fascioliasis emergence in southern Asia: Complete nuclear rDNA spacer and mtDNA gene sequences prove Indian patient infection related to fluke hybridization in northeastern India and Bangladesh[J],2024,18.
APA	Bargues, M. Dolores.,Artigas, Patricio.,Varghese, George M..,John, T. Jacob.,Ajjampur, Sitara S. R..,...&Mas-Coma, Santiago.(2024).Human fascioliasis emergence in southern Asia: Complete nuclear rDNA spacer and mtDNA gene sequences prove Indian patient infection related to fluke hybridization in northeastern India and Bangladesh.ONE HEALTH,18.
MLA	Bargues, M. Dolores,et al."Human fascioliasis emergence in southern Asia: Complete nuclear rDNA spacer and mtDNA gene sequences prove Indian patient infection related to fluke hybridization in northeastern India and Bangladesh".ONE HEALTH 18(2024).