气候变化领域集成服务门户(CCPortal): Life cycle assessment for early-stage process optimization of microbial biosurfactant production using kinetic models-a case study on mannosylerythritol lipids (MEL)

CCPortal

DOI	10.3389/fbioe.2024.1347452
	Life cycle assessment for early-stage process optimization of microbial biosurfactant production using kinetic models-a case study on mannosylerythritol lipids (MEL)
	Bippus, Lars; Briem, Ann-Kathrin; Beck, Alexander; Zibek, Susanne; Albrecht, Stefan
发表日期	2024
ISSN	2296-4185
起始页码	12
卷号	12
英文摘要	Introduction: This study assesses the environmental impacts of mannosylerythritol lipids (MELs) production for process optimization using life cycle assessment (LCA). MELs are glycolipid-type microbial biosurfactants with many possible applications based on their surface-active properties. They are generally produced by fungi from the family of Ustilaginaceae via fermentation in aerated bioreactors. The aim of our work is to accompany the development of biotechnological products at an early stage to enable environmentally sustainable process optimization.Methods: This is done by identifying hotspots and potentials for improvement based on a reliable quantification of the environmental impacts. The production processes of MELs are evaluated in a cradle-to-gate approach using the Environmental Footprint (EF) 3.1 impact assessment method. The LCA model is based on upscaled experimental data for the fermentation and purification, assuming the production at a 10 m(3) scale. In the case analyzed, MELs are produced from rapeseed oil and glucose, and purified by separation, solvent extraction, and chromatography.Results: The results of the LCA show that the provision of substrates is a major source of environmental impacts and accounts for 20% of the impacts on Climate Change and more than 70% in the categories Acidification and Eutrophication. Moreover, 33% of the impacts on Climate Change is caused by the energy requirements for aeration of the bioreactor, while purification accounts for 42% of the impacts respectively. For the purification, solvents are identified as the main contributors in most impact categories.Discussion: The results illustrate the potentials for process optimization to reduce the environmental impacts of substrate requirements, enhanced bioreactor aeration, and efficient solvent use in downstream processing. By a scenario analysis, considering both experimental adaptations and prospective variations of the process, the laboratory development can be supported with further findings and hence efficiently optimized towards environmental sustainability. Moreover, the presentation of kinetic LCA results over the fermentation duration shows a novel way of calculating and visualizing results that corresponds to the way of thinking of process engineers using established environmental indicators and a detailed system analysis. Altogether, this LCA study supports and demonstrates the potential for further improvements towards more environmentally friendly produced surfactants.
英文关键词	biosurfactant; mannosylerythritol lipids (MELs); life cycle assessment (LCA); fermentation; downstream processing; biotechnology; prospective LCA
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
WOS类目	Biotechnology & Applied Microbiology ; Engineering, Biomedical
WOS记录号	WOS:001177194300001
来源期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/291561
作者单位	University of Stuttgart; Fraunhofer Gesellschaft; Fraunhofer Gesellschaft; University of Stuttgart
推荐引用方式 GB/T 7714	Bippus, Lars,Briem, Ann-Kathrin,Beck, Alexander,et al. Life cycle assessment for early-stage process optimization of microbial biosurfactant production using kinetic models-a case study on mannosylerythritol lipids (MEL)[J],2024,12.
APA	Bippus, Lars,Briem, Ann-Kathrin,Beck, Alexander,Zibek, Susanne,&Albrecht, Stefan.(2024).Life cycle assessment for early-stage process optimization of microbial biosurfactant production using kinetic models-a case study on mannosylerythritol lipids (MEL).FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,12.
MLA	Bippus, Lars,et al."Life cycle assessment for early-stage process optimization of microbial biosurfactant production using kinetic models-a case study on mannosylerythritol lipids (MEL)".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 12(2024).