气候变化领域集成服务门户(CCPortal): Transcriptomic responses to drought stress in the Patagonian southern beech Nothofagus alpina

CCPortal

DOI	10.1186/s13717-024-00496-7
	Transcriptomic responses to drought stress in the Patagonian southern beech Nothofagus alpina
	Laphitz, Rita Maria Lopez; Arana, Maria Veronica; Varela, Santiago Agustin; Becker, Leandro Anibal; Soliani, Carolina; Azpilicueta, Maria Marta; Marchelli, Paula; Bellora, Nicolas
发表日期	2024
EISSN	2192-1709
起始页码	13
结束页码	1
卷号	13 期号:1
英文摘要	Background Deciphering the genetic architecture of drought tolerance could allow the candidate genes identification responding to water stress. In the Andean Patagonian forest, the genus Nothofagus represents an ecologically relevant species to be included in different genomic studies. These studies are scarce in South American ecosystems however represent an important source of genomic data in order to interpret future climate-change environment scenarios of these emblematic forests. Here, we achieved the assemblage of the transcriptome of N. alpina while searching for key genes of activated or suppressed metabolic pathways in response to drought stress. Results De novo transcriptome assembly resulted in 104,030 transcripts. Following confirmation of drought conditions, based on reduction of leaf water potential and stomatal conductance, a differential gene expression analysis resulted in 2720 significantly expressed genes (1601 up-regulated and 1119 down-regulated). Enrichment analysis (over-representation analysis and gene set enrichment analysis) resulted in more than one hundred stress-responsive term ontologies (i.e. biological processes) and pathways. Terms such as response to abscisic acid and pathways such as plant hormone signal transduction or starch and sucrose metabolism were over-represented. Protein-protein interaction assessment resulted in networks with significantly expressed top common hub gene clusters (e.g. plant-type cell wall biogenesis among down-regulated or ABA-signalling among up-regulated). These networks evidenced important regulators at gene expression such as transcriptional factors. Conclusions Responses of N. alpina seedlings to drought stress were evidenced by the activation of several genes linked to GO biological processes and KEGG pathways, which were mainly based on over-expression of specific protein kinases, phosphatases, synthases and transcription factors. This suggests an up-regulation of signalling pathways, triggered through plant hormones such as abscisic acid or auxin, which could counteract the osmotic stress created as a probable immediate response to drought. On the other hand, groups of carbon fixation genes related to the galactose metabolism, photosynthesis, secondary wall biogenesis, and fatty acid biosynthesis degradation were down-regulated under drought. Overall, our results provide new genomic data for understanding how non-model long-lived trees of Patagonian forests would acclimate to environmental changes.
英文关键词	Climate change; Differential gene expression; Hub genes; Patagonia; Temperate forest
语种	英语
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Ecology ; Environmental Sciences
WOS记录号	WOS:001214927000001
来源期刊	ECOLOGICAL PROCESSES
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/291433
作者单位	Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
推荐引用方式 GB/T 7714	Laphitz, Rita Maria Lopez,Arana, Maria Veronica,Varela, Santiago Agustin,et al. Transcriptomic responses to drought stress in the Patagonian southern beech Nothofagus alpina[J],2024,13(1).
APA	Laphitz, Rita Maria Lopez.,Arana, Maria Veronica.,Varela, Santiago Agustin.,Becker, Leandro Anibal.,Soliani, Carolina.,...&Bellora, Nicolas.(2024).Transcriptomic responses to drought stress in the Patagonian southern beech Nothofagus alpina.ECOLOGICAL PROCESSES,13(1).
MLA	Laphitz, Rita Maria Lopez,et al."Transcriptomic responses to drought stress in the Patagonian southern beech Nothofagus alpina".ECOLOGICAL PROCESSES 13.1(2024).