气候变化领域集成服务门户(CCPortal): Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts

CCPortal

DOI	10.3390/ijms25073677
	Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts
	Sola, Ivana; Gmizic, Daria; Pinteric, Marija; Tot, Ana; Ludwig-Mueller, Jutta
发表日期	2024
ISSN	1661-6596
EISSN	1422-0067
起始页码	25
结束页码	7
卷号	25 期号:7
英文摘要	Climate change causes shifts in temperature patterns, and plants adapt their chemical content in order to survive. We compared the effect of low (LT) and high (HT) growing temperatures on the phytochemical content of broccoli (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) microgreens and the bioactivity of their extracts. Using different spectrophotometric, LC-MS/MS, GC-MS, and statistical methods, we found that LT increased the total phenolics and tannins in broccoli. The total glucosinolates were also increased by LT; however, they were decreased by HT. Soluble sugars, known osmoprotectants, were increased by both types of stress, considerably more by HT than LT, suggesting that HT causes a more intense osmotic imbalance. Both temperatures were detrimental for chlorophyll, with HT being more impactful than LT. HT increased hormone indole-3-acetic acid, implying an important role in broccoli's defense. Ferulic and sinapic acid showed a trade-off scheme: HT increased ferulic while LT increased sinapic acid. Both stresses decreased the potential of broccoli to act against H2O2 damage in mouse embryonal fibroblasts (MEF), human keratinocytes, and liver cancer cells. Among the tested cell types treated by H2O2, the most significant reduction in ROS (36.61%) was recorded in MEF cells treated with RT extracts. The potential of broccoli extracts to inhibit alpha-amylase increased following both temperature stresses; however, the inhibition of pancreatic lipase was increased by LT only. From the perspective of nutritional value, and based on the obtained results, we conclude that LT conditions result in more nutritious broccoli microgreens than HT.
英文关键词	auxins; Brassicaceae; climate change; metabolic response; microgreens; photosynthetic pigments; polyphenolics; ROS; temperature stress; vitamin C
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001200976800001
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/289118
作者单位	University of Zagreb; Rudjer Boskovic Institute; University of Zagreb; Technische Universitat Dresden
推荐引用方式 GB/T 7714	Sola, Ivana,Gmizic, Daria,Pinteric, Marija,et al. Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts[J],2024,25(7).
APA	Sola, Ivana,Gmizic, Daria,Pinteric, Marija,Tot, Ana,&Ludwig-Mueller, Jutta.(2024).Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(7).
MLA	Sola, Ivana,et al."Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.7(2024).