气候变化领域集成服务门户(CCPortal): Non-concordant epigenetic and transcriptional responses to acute thermal stress in western mosquitofish (Gambusia affinis)

CCPortal

DOI	10.1111/mec.17332
	Non-concordant epigenetic and transcriptional responses to acute thermal stress in western mosquitofish (Gambusia affinis)
	Ren, Xingyue; Zhao, Junjie; Hu, Juntao
发表日期	2024
ISSN	0962-1083
EISSN	1365-294X
英文摘要	Climate change is intensifying the frequency and severity of extreme temperatures. Understanding the molecular mechanisms underlying the ability to cope with acute thermal stress is key for predicting species' responses to extreme temperature events. While many studies have focused on the individual roles of gene expression, post-transcriptional processes and epigenetic modifications in response to acute thermal stress, the relative contribution of these molecular mechanisms remains unclear. The wide range of thermal limits of western mosquitofish (Gambusia affinis) provides an opportunity to explore this interplay. Here, we quantified changes in gene expression, alternative splicing, DNA methylation and microRNA (miRNA) expression in muscle tissue dissected from mosquitofish immediately after reaching high (CTmax) or low thermal limit (CTmin). Although the numbers of genes showing expression and splicing changes in response to acute temperature stress were small, we found a possibly larger and non-redundant role of splicing compared to gene expression, with more genes being differentially spliced (DSGs) than differentially expressed (DEGs), and little overlap between DSGs and DEGs. We also identified a small proportion of CpGs showing significant methylation change (i.e. differentially methylated cytosines, DMCs) in fish at thermal limits; however, there was no overlap between DEGs and genes annotated with DMCs in both CTmax and CTmin experiments. The weak interplay between epigenetic modifications and gene expression was further supported by our discoveries of no differentially expressed miRNAs. These findings provide novel insights into the relative role of different molecular mechanisms underlying immediate responses to extreme temperatures and demonstrate non-concordant responses of epigenetic and transcriptional mechanisms to acute temperature stress.
英文关键词	acute thermal stress; alternative splicing; DNA methylation; Gambusia affinis; gene expression; microRNA
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号	WOS:001190645400001
来源期刊	MOLECULAR ECOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/288957
作者单位	Fudan University; Fudan University
推荐引用方式 GB/T 7714	Ren, Xingyue,Zhao, Junjie,Hu, Juntao. Non-concordant epigenetic and transcriptional responses to acute thermal stress in western mosquitofish (Gambusia affinis)[J],2024.
APA	Ren, Xingyue,Zhao, Junjie,&Hu, Juntao.(2024).Non-concordant epigenetic and transcriptional responses to acute thermal stress in western mosquitofish (Gambusia affinis).MOLECULAR ECOLOGY.
MLA	Ren, Xingyue,et al."Non-concordant epigenetic and transcriptional responses to acute thermal stress in western mosquitofish (Gambusia affinis)".MOLECULAR ECOLOGY (2024).