气候变化领域集成服务门户(CCPortal): Insights into the mechanism underlying UV-B induced flavonoid metabolism in callus of a Tibetan medicinal plant Mirabilis himalaica

CCPortal

DOI	10.1016/j.jplph.2023.154074
	Insights into the mechanism underlying UV-B induced flavonoid metabolism in callus of a Tibetan medicinal plant Mirabilis himalaica
	Li, Rongchen; Liu, Huan; Liu, Yanjing; Guo, Jiaojiao; Chen, Yuzhen; Lan, Xiaozhong; Lu, Cunfu
发表日期	2023
ISSN	0176-1617
EISSN	1618-1328
卷号	288
英文摘要	Mirabilis himalaica is an important Tibetan medicinal plant in China. However, it has become a rare and class I endangered Tibetan medicine plant. Therefore, the use of callus to propagate germplasm resources is of great significance. We found that the flavonoid content of M. himalaica callus increased continuously with the extension of UV-B treatment. Multi-omics profiles were used to reveal the co-expression patterns of gene networks of flavonoid metabolism in M. himalaica callus during UV-B radiation. Results showed that five medicinal metabolics, including geranin, eriodictyol, astragalin, isoquercetin, pyrotechnic acid, and one anthocyanin malvide-3-O-glucoside were identified. The transcriptome data were divided into 46 modules according to the expression pattern by WGCNA (weighted gene co-expression network analysis), of which the module Turquoise had the strongest correlation with six target metabolites. We found that seven structural genes and twenty-five transcription factors were related to the metabolism of flavonoid synthesis, among which the structural genes CHI, C4H and UGT79B6 had strong co-expression relationships with the 6 target metabolites. WRKY42, WRKY7, bHLH128 and other transcription factors had strong co-expression relationships with multiple structural genes. Consequently, these findings suggest callus grown under UV-B treatment could be an effective alternative medical resource of M. himalaica, which is valuable for conservation and usage of this wild and endangered plant.
关键词	Multi-omics Flavonoids Ultraviolet-B Metabolic pathways WGCNA
英文关键词	TRANSCRIPTOMIC ANALYSIS REVEALS; CHALCONE ISOMERASE GENE; CINNAMATE 4-HYDROXYLASE; MOLECULAR-CLONING; BIOSYNTHESIS; RADIATION; ACCUMULATION; ANTHOCYANIN; EXPRESSION; PATHWAYS
WOS研究方向	Plant Sciences
WOS记录号	WOS:001068934300001
来源期刊	JOURNAL OF PLANT PHYSIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283674
作者单位	Beijing Forestry University; Tibet Agriculture & Animal Husbandry University
推荐引用方式 GB/T 7714	Li, Rongchen,Liu, Huan,Liu, Yanjing,et al. Insights into the mechanism underlying UV-B induced flavonoid metabolism in callus of a Tibetan medicinal plant Mirabilis himalaica[J],2023,288.
APA	Li, Rongchen.,Liu, Huan.,Liu, Yanjing.,Guo, Jiaojiao.,Chen, Yuzhen.,...&Lu, Cunfu.(2023).Insights into the mechanism underlying UV-B induced flavonoid metabolism in callus of a Tibetan medicinal plant Mirabilis himalaica.JOURNAL OF PLANT PHYSIOLOGY,288.
MLA	Li, Rongchen,et al."Insights into the mechanism underlying UV-B induced flavonoid metabolism in callus of a Tibetan medicinal plant Mirabilis himalaica".JOURNAL OF PLANT PHYSIOLOGY 288(2023).