气候变化领域集成服务门户(CCPortal): Different Responses of Bacteria and Archaea to Environmental Variables in Brines of the Mahai Potash Mine, Qinghai-Tibet Plateau

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DOI	10.3390/microorganisms11082002
	Different Responses of Bacteria and Archaea to Environmental Variables in Brines of the Mahai Potash Mine, Qinghai-Tibet Plateau
	Xie, Linglu; Yu, Shan; Lu, Xindi; Liu, Siwei; Tang, Yukai; Lu, Hailong
发表日期	2023
EISSN	2076-2607
卷号	11 期号:8
英文摘要	Salt mines feature both autochthonous and allochthonous microbial communities introduced by industrialization. It is important to generate the information on the diversity of the microbial communities present in the salt mines and how they are shaped by the environment representing ecological diversification. Brine from Mahai potash mine (Qianghai, China), an extreme hypersaline environment, is used to produce potash salts for hundreds of millions of people. However, halophiles preserved in this niche during deposition are still unknown. In this study, using high-throughput 16S rRNA gene amplicon sequencing and estimation of physicochemical variables, we examined brine samples collected from locations with the gradient of industrial activity intensity and discrete hydrochemical compositions in the Mahai potash mine. Our findings revealed a highly diverse bacterial community, mainly composed of Pseudomonadota in the hypersaline brines from the industrial area, whereas in the natural brine collected from the upstream Mahai salt lake, most of the 16S rRNA gene reads were assigned to Bacteroidota. Halobacteria and halophilic methanogens dominated archaeal populations. Furthermore, we discovered that in the Mahai potash mining area, bacterial communities tended to respond to anthropogenic influences. In contrast, archaeal diversity and compositions were primarily shaped by the chemical properties of the hypersaline brines. Conspicuously, distinct methanogenic communities were discovered in sets of samples with varying ionic compositions, indicating their strong sensitivity to the brine hydrochemical alterations. Our findings provide the first taxonomic snapshot of microbial communities from the Mahai potash mine and reveal the different responses of bacteria and archaea to environmental variations in this high-altitude aquatic ecosystem.
关键词	microbial diversity microbial response potash mine brine hydrochemistry halophilic methanogens Illumina Novaseq sequencing
英文关键词	COMMUNITY COMPOSITION; SALT LAKE; SP NOV.; HYPERSALINE ENVIRONMENTS; FLOTATION ENRICHMENT; MICROBIAL DIVERSITY; SALINITY; MICROORGANISMS; METHANOGEN; DYNAMICS
WOS研究方向	Microbiology
WOS记录号	WOS:001055733300001
来源期刊	MICROORGANISMS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283505
作者单位	Peking University; Peking University
推荐引用方式 GB/T 7714	Xie, Linglu,Yu, Shan,Lu, Xindi,et al. Different Responses of Bacteria and Archaea to Environmental Variables in Brines of the Mahai Potash Mine, Qinghai-Tibet Plateau[J],2023,11(8).
APA	Xie, Linglu,Yu, Shan,Lu, Xindi,Liu, Siwei,Tang, Yukai,&Lu, Hailong.(2023).Different Responses of Bacteria and Archaea to Environmental Variables in Brines of the Mahai Potash Mine, Qinghai-Tibet Plateau.MICROORGANISMS,11(8).
MLA	Xie, Linglu,et al."Different Responses of Bacteria and Archaea to Environmental Variables in Brines of the Mahai Potash Mine, Qinghai-Tibet Plateau".MICROORGANISMS 11.8(2023).