气候变化领域集成服务门户(CCPortal): Are reactive oxygen species (ROS) a suitable metric to predict toxicity of carbonaceous aerosol particles?

CCPortal

DOI	10.5194/acp-22-1793-2022
	Are reactive oxygen species (ROS) a suitable metric to predict toxicity of carbonaceous aerosol particles?
	Zhang, Zhi-Hui; Hartner, Elena; Utinger, Battist; Gfeller, Benjamin; Paul, Andreas; Sklorz, Martin; Czech, Hendryk; Yang, Bin Xia; Su, Xin Yi; Jakobi, Gert; Orasche, Jurgen; Schnelle-Kreis, Juergen; Jeong, Seongho; Groeger, Thomas; Pardo, Michal; Hohaus, Thorsten; Adam, Thomas; Kiendler-Scharr, Astrid; Rudich, Yinon; Zimmermann, Ralf; Kalberer, Markus
发表日期	2022
ISSN	1680-7316
EISSN	1680-7324
起始页码	1793
结束页码	1809
卷号	22 期号:3 页码:17
英文摘要	It is being suggested that particle-bound or particle-induced reactive oxygen species (ROS), which significantly contribute to the oxidative potential (OP) of aerosol particles, are a promising metric linking aerosol compositions to toxicity and adverse health effects. However, accurate ROS quantification remains challenging due to the reactive and short-lived nature of many ROS components and the lack of appropriate analytical methods for a reliable quantification. Consequently, it remains difficult to gauge their impact on human health, especially to identify how aerosol particle sources and atmospheric processes drive particle-bound ROS formation in a real-world urban environment. In this study, using a novel online particle-bound ROS instrument (OPROSI), we comprehensively characterized and compared the formation of ROS in secondary organic aerosols (SOAs) generated from organic compounds that represent anthropogenic (naphthalene, SOA(NAP)) and biogenic (beta-pinene, SOA(beta PIN)) precursors. The SOA mass was condensed onto soot particles (SP) under varied atmospherically relevant conditions (photochemical aging and humidity) to mimic the SOA formation from a mixing of traffic-related carbonaceous primary aerosols and volatile organic compounds (VOCs). We systematically analyzed the ability of the aqueous extracts of the two aerosol types (SOA(NAP)-SP and SOA(beta PIN)-SP) to induce ROS production and OP. We further investigated cytotoxicity and cellular ROS production after exposing human lung epithelial cell cultures (A549) to extracts of the two aerosols. A significant finding of this study is that more than 90% of all ROS components in both SOA types have a short lifetime, highlighting the need to develop online instruments for a meaningful quantification of ROS. Our results also show that photochemical aging promotes particle-bound ROS production and enhances the OP of the aerosols. Compared to SOA(beta PIN)-SP, SOA(NAP)-SP elicited a higher acellular and cellular ROS production, a higher OP, and a lower cell viability. These consistent results between chemical-based and biological-based analyses indicate that particle-bound ROS quantification could be a feasible metric to predict aerosol particle toxicity and adverse human effects. Moreover, the cellular ROS production caused by SOA exposure not only depends on aerosol type but is also affected by exposure dose, highlighting a need to mimic the process of particle deposition onto lung cells and their interactions as realistically as possible to avoid unknown biases.
学科领域	Environmental Sciences; Meteorology & Atmospheric Sciences
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Meteorology & Atmospheric Sciences
WOS记录号	WOS:000758104200001
来源期刊	ATMOSPHERIC CHEMISTRY AND PHYSICS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/273722
作者单位	University of Basel; University of Cambridge; Helmholtz Association; Helmholtz-Center Munich - German Research Center for Environmental Health; University of Rostock; Agency for Science Technology & Research (ASTAR); ASTAR - Institute of Molecular & Cell Biology (IMCB); Weizmann Institute of Science; Bundeswehr University Munich
推荐引用方式 GB/T 7714	Zhang, Zhi-Hui,Hartner, Elena,Utinger, Battist,et al. Are reactive oxygen species (ROS) a suitable metric to predict toxicity of carbonaceous aerosol particles?[J],2022,22(3):17.
APA	Zhang, Zhi-Hui.,Hartner, Elena.,Utinger, Battist.,Gfeller, Benjamin.,Paul, Andreas.,...&Kalberer, Markus.(2022).Are reactive oxygen species (ROS) a suitable metric to predict toxicity of carbonaceous aerosol particles?.ATMOSPHERIC CHEMISTRY AND PHYSICS,22(3),17.
MLA	Zhang, Zhi-Hui,et al."Are reactive oxygen species (ROS) a suitable metric to predict toxicity of carbonaceous aerosol particles?".ATMOSPHERIC CHEMISTRY AND PHYSICS 22.3(2022):17.