气候变化领域集成服务门户(CCPortal): Expression profiles of glucosinolate biosynthetic genes in turnip (Brassica rapa var. rapa) at different developmental stages and effect of transformed flavin-containing monooxygenase genes on hairy root glucosinolate content

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DOI	10.1002/jsfa.10111
	Expression profiles of glucosinolate biosynthetic genes in turnip (Brassica rapa var. rapa) at different developmental stages and effect of transformed flavin-containing monooxygenase genes on hairy root glucosinolate content
	Yang, Ya; Hu, Yue; Yue, Yanling; Pu, Yanan; Yin, Xin; Duan, Yuanwen; Huang, Aixia; Yang, Yunqiang; Yang, Yongping
通讯作者	Yang, YQ (通讯作者)
发表日期	2020
ISSN	0022-5142
EISSN	1097-0010
起始页码	1064
结束页码	1071
卷号	100 期号:3
英文摘要	BACKGROUND Glucosinolates (GSLs) are secondary metabolites, mainly existing in Brassica vegetables. Their breakdown products have health benefits and contribute to the distinctive taste of these vegetables. Because of their high value, there is a lot of interest in developing breeding strategies to increase the content of beneficial GSLs in Brassica species. GSLs are synthesized from certain amino acids and their biological roles depend largely on the structure of their side chains. Flavin-containing monooxygenase (FMOGS-OX) genes are involved in the synthesis of these side chains. To better understand GSL biosynthesis, we sequenced the transcriptomes of turnip (Brassica rapa var. rapa) tubers at four developmental stages (S1-S4) and determined their GSL content. RESULTS The total GSL content was high at the early stage (S1) of tuber development and increased up to S3, then decreased at S4. We detected 61 differentially expressed genes, including five FMOGS-OX genes, that were related for GSL biosynthesis among the four developmental stages. Most of these genes were highly expressed at stages S1 to S3, but their expression was much lower at S4. We estimated the effect of the five FMOGS-OX genes on GSL content by overexpressing them in turnip hairy roots and found that the amount of aliphatic GSLs increased significantly in the transgenic plants. CONCLUSION The transcriptome data and characterization of genes involved in GSL biosynthesis, particularly the FMOGS-OX genes, will be valuable for improving the yield of beneficial GSLs in turnip and other Brassica crops. (c) 2019 Society of Chemical Industry
关键词	CHINESE-CABBAGE ORGANS ISOTHIOCYANATES IDENTIFICATION HYDROLYSIS COLLECTION METABOLISM VEGETABLES CULTIVARS DIVERSITY
英文关键词	Brassica; flavin-containing monooxygenase; hairy root; transcriptome; tuber developmental stage; turnip
语种	英语
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:000500588000001
来源期刊	JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259969
推荐引用方式 GB/T 7714	Yang, Ya,Hu, Yue,Yue, Yanling,et al. Expression profiles of glucosinolate biosynthetic genes in turnip (Brassica rapa var. rapa) at different developmental stages and effect of transformed flavin-containing monooxygenase genes on hairy root glucosinolate content[J]. 中国科学院青藏高原研究所,2020,100(3).
APA	Yang, Ya.,Hu, Yue.,Yue, Yanling.,Pu, Yanan.,Yin, Xin.,...&Yang, Yongping.(2020).Expression profiles of glucosinolate biosynthetic genes in turnip (Brassica rapa var. rapa) at different developmental stages and effect of transformed flavin-containing monooxygenase genes on hairy root glucosinolate content.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,100(3).
MLA	Yang, Ya,et al."Expression profiles of glucosinolate biosynthetic genes in turnip (Brassica rapa var. rapa) at different developmental stages and effect of transformed flavin-containing monooxygenase genes on hairy root glucosinolate content".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 100.3(2020).