气候变化领域集成服务门户(CCPortal): Salinity Affects the Composition of the Aerobic Methanotroph Community in Alkaline Lake Sediments from the Tibetan Plateau

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DOI	10.1007/s00248-016-0879-5
	Salinity Affects the Composition of the Aerobic Methanotroph Community in Alkaline Lake Sediments from the Tibetan Plateau
	Deng, Yongcui; Liu, Yongqin; Dumont, Marc; Conrad, Ralf
通讯作者	Liu, YQ (通讯作者)
发表日期	2017
ISSN	0095-3628
EISSN	1432-184X
起始页码	101
结束页码	110
卷号	73 期号:1
英文摘要	Lakes are widely distributed on the Tibetan Plateau, which plays an important role in natural methane emission. Aerobic methanotrophs in lake sediments reduce the amount of methane released into the atmosphere. However, no study to date has analyzed the methanotroph community composition and their driving factors in sediments of these high-altitude lakes (> 4000 m). To provide new insights on this aspect, the abundance and composition in the sediments of six high-altitude alkaline lakes (including both freshwater and saline lakes) on the Tibetan Plateau were studied. The quantitative PCR, terminal restriction fragment length polymorphism, and 454-pyrosequencing methods were used to target the pmoA genes. The pmoA gene copies ranged 10(4)-10(6) per gram fresh sediment. Type I methanotrophs predominated in Tibetan lake sediments, with Methylobacter and uncultivated type Ib methanotrophs being dominant in freshwater lakes and Methylomicrobium in saline lakes. Combining the pmoA-pyrosequencing data from Tibetan lakes with other published pmoA-sequencing data from lake sediments of other regions, a significant salinity and alkalinity effect (P = 0.001) was detected, especially salinity, which explained similar to 25% of methanotroph community variability. The main effect was Methylomicrobium being dominant (up to 100%) in saline lakes only. In freshwater lakes, however, methanotroph composition was relatively diverse, including Methylobacter, Methylocystis, and uncultured type Ib clusters. This study provides the first methanotroph data for high-altitude lake sediments (> 4000 m) and shows that salinity is a driving factor for the community composition of aerobic methanotrophs.
关键词	16S RIBOSOMAL-RNA METHANE OXIDATION MESSENGER-RNA ZOIGE WETLAND LONAR LAKE SODA LAKES MONO LAKE DIVERSITY BACTERIA PMOA
英文关键词	Aerobic methanotrophs; High-altitude lake; Methylomicrobium; Salinity; Tibetan Plateau
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
WOS类目	Ecology ; Marine & Freshwater Biology ; Microbiology
WOS记录号	WOS:000392130400010
来源期刊	MICROBIAL ECOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259051
推荐引用方式 GB/T 7714	Deng, Yongcui,Liu, Yongqin,Dumont, Marc,et al. Salinity Affects the Composition of the Aerobic Methanotroph Community in Alkaline Lake Sediments from the Tibetan Plateau[J]. 中国科学院青藏高原研究所,2017,73(1).
APA	Deng, Yongcui,Liu, Yongqin,Dumont, Marc,&Conrad, Ralf.(2017).Salinity Affects the Composition of the Aerobic Methanotroph Community in Alkaline Lake Sediments from the Tibetan Plateau.MICROBIAL ECOLOGY,73(1).
MLA	Deng, Yongcui,et al."Salinity Affects the Composition of the Aerobic Methanotroph Community in Alkaline Lake Sediments from the Tibetan Plateau".MICROBIAL ECOLOGY 73.1(2017).