气候变化领域集成服务门户(CCPortal): Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling

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DOI	10.3390/ijms17060966
	Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling
	Zhou, Yanli; Sun, Xudong; Yang, Yunqiang; Li, Xiong; Cheng, Ying; Yang, Yongping
通讯作者	Yang, YP (通讯作者)
发表日期	2016
EISSN	1422-0067
卷号	17 期号:6
英文摘要	Stipa purpurea (S. purpurea) is the dominant plant species in the alpine steppe of the Qinghai-Tibet Plateau, China. It is highly resistant to cold and drought conditions. However, the underlying mechanisms regulating the stress tolerance are unknown. In this study, a CIPK gene from S. purpurea (SpCIPK26) was isolated. The SpCIPK26 coding region consisted of 1392 bp that encoded 464 amino acids. The protein has a highly conserved catalytic structure and regulatory domain. The expression of SpCIPK26 was induced by drought and salt stress. SpCIPK26 overexpression in Arabidopsis thaliana (A. thaliana) plants provided increased tolerance to drought and salt stress in an abscisic acid (ABA)-dependent manner. Compared with wild-type A. thaliana plants, SpCIPK26-overexpressing plants had higher survival rates, water potentials, and photosynthetic efficiency (Fv/Fm), as well as lower levels of reactive oxygen species (ROS) following exposure to drought and salt stress. Gene expression analyses indicated stress-inducible genes (RD29A, RD29B, and ABF2) and a ROS-scavenger gene (CAT1) were upregulated in SpCIPK26-overexpressing plants after stress treatments. All of these marker genes are associated with ABA-responsive cis-acting elements. Additionally, the similarities in the gene expression patterns following ABA, mannitol, and NaCl treatments suggest SpCIPK26 has an important role during plant responses to drought and salt stress and in regulating ABA signaling.
关键词	PROTEIN-KINASES STRESS TOLERANCE STIPA-PURPUREA ABIOTIC STRESS ELECTROLYTE LEAKAGE CALCIUM SENSORS OSMOTIC-STRESS PLANT-RESPONSE WATER-STRESS ABA
英文关键词	CIPK; Stipa purpurea; salt; drought; ABA; ROS
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000378799300178
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258756
推荐引用方式 GB/T 7714	Zhou, Yanli,Sun, Xudong,Yang, Yunqiang,et al. Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling[J]. 中国科学院青藏高原研究所,2016,17(6).
APA	Zhou, Yanli,Sun, Xudong,Yang, Yunqiang,Li, Xiong,Cheng, Ying,&Yang, Yongping.(2016).Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(6).
MLA	Zhou, Yanli,et al."Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.6(2016).