气候变化领域集成服务门户(CCPortal): Isolation and Functional Analysis of SpWOX13 from Stipa purpurea

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DOI	10.1007/s11105-014-0848-4
	Isolation and Functional Analysis of SpWOX13 from Stipa purpurea
	Sun, Xudong; Xiang, Nan; Wang, Chongde; Yang, Shihai; Li, Xiong; Yang, Yunqiang; Yang, Yongping
通讯作者	Yang, YQ (通讯作者)
发表日期	2015
ISSN	0735-9640
EISSN	1572-9818
起始页码	1441
结束页码	1450
卷号	33 期号:5
英文摘要	Members of the WUSCHEL-related homeobox (WOX) gene family encode plant-specific transcription factors that play key roles in controlling gene expression at all stages of plant development. In the present study, we isolated and characterized SpWOX13 (GenBank accession no. KJ713172), a member of the WOX gene family, from Stipa purpurea. Sequence and phylogenetic analyses showed that SpWOX13 belongs to an ancient clade of WOX genes. A subcellular localization analysis indicated that SpWOX13 localizes in the nucleus. The transcript levels of SpWOX13 were higher in young leaves and the vegetative apex than in other tissues of S. purpurea. Ectopic expression of SpWOX13 in Arabidopsis resulted in late flowering plants as these showed more rosette leaves. Histological analyses of the transgenic plants showed aberrant arrangements in the tunica cell layers of the shoot apical meristem. Scanning electron microscopy analyses revealed that the leaf pavement cells were larger in SpWOX13-overexpressing transgenic plants than in wild type. Our results suggest that SpWOX13 is involved in delaying and/or inhibiting cell differentiation.
关键词	HOMEOBOX GENE CELL FATE SHOOT WUSCHEL LEAF PROLIFERATION ACTIVATION TRANSITION EXPRESSION GROWTH
英文关键词	SpWOX13; Transcription factor; Cell differentiation; Stipa purpurea; Arabidopsis
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemical Research Methods ; Plant Sciences
WOS记录号	WOS:000360392800022
来源期刊	PLANT MOLECULAR BIOLOGY REPORTER
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258528
推荐引用方式 GB/T 7714	Sun, Xudong,Xiang, Nan,Wang, Chongde,et al. Isolation and Functional Analysis of SpWOX13 from Stipa purpurea[J]. 中国科学院青藏高原研究所,2015,33(5).
APA	Sun, Xudong.,Xiang, Nan.,Wang, Chongde.,Yang, Shihai.,Li, Xiong.,...&Yang, Yongping.(2015).Isolation and Functional Analysis of SpWOX13 from Stipa purpurea.PLANT MOLECULAR BIOLOGY REPORTER,33(5).
MLA	Sun, Xudong,et al."Isolation and Functional Analysis of SpWOX13 from Stipa purpurea".PLANT MOLECULAR BIOLOGY REPORTER 33.5(2015).