气候变化领域集成服务门户(CCPortal): Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient

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DOI	10.1371/journal.pone.0117475
	Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient
	Yang, Yunqiang; Dong, Chao; Yang, Shihai; Li, Xiong; Sun, Xudong; Yang, Yongping
通讯作者	Sun, XD (通讯作者)
发表日期	2015
ISSN	1932-6203
卷号	10 期号:2
英文摘要	Stipa purpurea, an endemic forage species on the Tibetan Plateau, is highly resistant to cold and drought, but the mechanisms underlying its responses to drought stress remain elusive. An understanding of such mechanisms may be useful for developing cultivars that are adaptable to water deficit. In this study, we analyzed the physiological and proteomic responses of S. purpurea under increasing drought stress. Seedlings of S. purpurea were subjected to a drought gradient in a controlled experiment, and proteins showing changes in abundance under these conditions were identified by two-dimensional electrophoresis followed by mass spectrometry analysis. A western blotting analysis was conducted to confirm the increased abundance of a heat-shock protein, NCED2, and a dehydrin in S. purpurea seedlings under drought conditions. We detected carbonylated proteins to identify oxidation-sensitive proteins in S. purpurea seedlings, and found that ribulose-1, 5-bisphosphate carboxylase oxygenase (RuBisCO) was one of the oxidation-sensitive proteins under drought. Together, these results indicated drought stress might inhibit photosynthesis in S. purpurea by oxidizing RuBisCO, but the plants were able to maintain photosynthetic efficiency by a compensatory upregulation of unoxidized RuBisCO and other photosynthesis-related proteins. Further analyses confirmed that increased abundance of antioxidant enzymes could balance the redox status of the plants to mitigate drought induced oxidative damage.
关键词	OSMOTIC ADJUSTMENT TIBETAN PLATEAU HEAT-STRESS ARABIDOPSIS RESPONSES PLANT ABA GROWTH ROOT CELL
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000348822600071
来源期刊	PLOS ONE
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258456
推荐引用方式 GB/T 7714	Yang, Yunqiang,Dong, Chao,Yang, Shihai,et al. Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient[J]. 中国科学院青藏高原研究所,2015,10(2).
APA	Yang, Yunqiang,Dong, Chao,Yang, Shihai,Li, Xiong,Sun, Xudong,&Yang, Yongping.(2015).Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient.PLOS ONE,10(2).
MLA	Yang, Yunqiang,et al."Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient".PLOS ONE 10.2(2015).