气候变化领域集成服务门户(CCPortal): Thermoflexus hugenholtzii gen. nov., sp nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov and Thermoflexales ord. nov.

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DOI	10.1099/ijs.0.055855-0
	Thermoflexus hugenholtzii gen. nov., sp nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov and Thermoflexales ord. nov.
	Dodsworth, Jeremy A.; Gevorkian, Jonathan; Despujos, Fairuz; Cole, Jessica K.; Murugapiran, Senthil K.; Ming, Hong; Li, Wen-Jun; Zhang, Gengxin; Dohnalkova, Alice; Hedlund, Brian P.
通讯作者	Dodsworth, JA (通讯作者)
发表日期	2014
ISSN	1466-5026
EISSN	1466-5034
起始页码	2119
结束页码	2127
卷号	64
英文摘要	A thermophilic, filamentous, heterotrophic bacterium, designated strain JAD2(T), a member of an as-yet uncultivated lineage that is present and sometimes abundant in some hot springs worldwide, was isolated from sediment of Great Boiling Spring in Nevada, USA. Cells had a mean diameter of 0.3 mu m and length of 4.0 mu m, and formed filaments that typically ranged in length from 20 to 200 mu m. Filaments were negative for the Gram stain reaction, spores were not formed and motility was not observed. The optimum temperature for growth was 72.5-75 degrees C, with a range of 67.5-75 degrees C, and the optimum pH for growth was 6.75, with a range of pH 6.5-7.75. Peptone, tryptone or yeast extract were able to support growth when supplemented with vitamins, but no growth was observed using a variety of defined organic substrates. Strain JAD2(T) was microaerophilic and facultatively anaerobic, with optimal growth at 1% (v/v) O-2 and an upper limit of 8% O-2. The major cellular fatty acids (>5%) were C-16:0, C-19:0, C-18:0, C-20:0 and C-19:1. The genomic DNA G+C content was 69.3 mol%. Phylogenetic and phylogenomic analyses using sequences of the 16S rRNA gene and other conserved genes placed JAD2(T) within the phylum Chloroflexi, but not within any existing class in this phylum. These results indicate that strain JAD2(T) is the first cultivated representative of a novel lineage within the phylum Chloroflexi, for which we propose the name Thermoflexus hugenholtzii gen. nov., sp. nov., within Thermoflexia classis nov., Thermoflexales ord. nov. and Thermoflexaceae fam. nov. The type strain of Thermoflexus hugenholtzii is JAD2(T) (=JCM 19131(T)=CCTCC AB-2014030(T)).
关键词	PHYLUM CHLOROFLEXI EMENDED DESCRIPTION HOT-SPRINGS PHYLOGENETIC TREES GREAT-BASIN FATTY-ACIDS DIVERSITY LINEAGE THERMOMICROBIUM ECOPHYSIOLOGY
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000340019200045
来源期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258396
推荐引用方式 GB/T 7714	Dodsworth, Jeremy A.,Gevorkian, Jonathan,Despujos, Fairuz,et al. Thermoflexus hugenholtzii gen. nov., sp nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov and Thermoflexales ord. nov.[J]. 中国科学院青藏高原研究所,2014,64.
APA	Dodsworth, Jeremy A..,Gevorkian, Jonathan.,Despujos, Fairuz.,Cole, Jessica K..,Murugapiran, Senthil K..,...&Hedlund, Brian P..(2014).Thermoflexus hugenholtzii gen. nov., sp nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov and Thermoflexales ord. nov..INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,64.
MLA	Dodsworth, Jeremy A.,et al."Thermoflexus hugenholtzii gen. nov., sp nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov and Thermoflexales ord. nov.".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64(2014).