气候变化领域集成服务门户(CCPortal): Ectopic activation of GABAB receptors inhibits neurogenesis and metamorphosis in the cnidarian Nematostella vectensis

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DOI	10.1038/s41559-020-01338-3
	Ectopic activation of GABAB receptors inhibits neurogenesis and metamorphosis in the cnidarian Nematostella vectensis
	Levy S.; Brekhman V.; Bakhman A.; Malik A.; Sebé-Pedrós A.; Kosloff M.; Lotan T.
发表日期	2021
ISSN	2397-334X
起始页码	111
结束页码	121
卷号	5 期号:1
英文摘要	The metabotropic gamma-aminobutyric acid B receptor (GABABR) is a G protein-coupled receptor that mediates neuronal inhibition by the neurotransmitter GABA. While GABABR-mediated signalling has been suggested to play central roles in neuronal differentiation and proliferation across evolution, it has mostly been studied in the mammalian brain. Here, we demonstrate that ectopic activation of GABABR signalling affects neurogenic functions in the sea anemone Nematostella vectensis. We identified four putative Nematostella GABABR homologues presenting conserved three-dimensional extracellular domains and residues needed for binding GABA and the GABABR agonist baclofen. Moreover, sustained activation of GABABR signalling reversibly arrests the critical metamorphosis transition from planktonic larva to sessile polyp life stage. To understand the processes that underlie the developmental arrest, we combined transcriptomic and spatial analyses of control and baclofen-treated larvae. Our findings reveal that the cnidarian neurogenic programme is arrested following the addition of baclofen to developing larvae. Specifically, neuron development and neurite extension were inhibited, resulting in an underdeveloped and less organized nervous system and downregulation of proneural factors including NvSoxB(2), NvNeuroD1 and NvElav1. Our results thus point to an evolutionarily conserved function of GABABR in neurogenesis regulation and shed light on early cnidarian development. ? 2020, The Author(s), under exclusive licence to Springer Nature Limited.
语种	英语
scopus关键词	4 aminobutyric acid; 4 aminobutyric acid B receptor; animal; genetics; metamorphosis; nervous system development; sea anemone; Animals; gamma-Aminobutyric Acid; Metamorphosis, Biological; Neurogenesis; Receptors, GABA-B; Sea Anemones
来源期刊	Nature Ecology & Evolution
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257042
作者单位	Marine Biology Department, The Leon H. Charney School of Marine Sciences, University of Haifa, Haifa, Israel; Department of Human Biology, University of Haifa, Haifa, Israel; Centre for Genomic Regulation, Barcelona Institute of Science and Technology, Universitat Pompeu Fabra, Barcelona, Spain
推荐引用方式 GB/T 7714	Levy S.,Brekhman V.,Bakhman A.,et al. Ectopic activation of GABAB receptors inhibits neurogenesis and metamorphosis in the cnidarian Nematostella vectensis[J],2021,5(1).
APA	Levy S..,Brekhman V..,Bakhman A..,Malik A..,Sebé-Pedrós A..,...&Lotan T..(2021).Ectopic activation of GABAB receptors inhibits neurogenesis and metamorphosis in the cnidarian Nematostella vectensis.Nature Ecology & Evolution,5(1).
MLA	Levy S.,et al."Ectopic activation of GABAB receptors inhibits neurogenesis and metamorphosis in the cnidarian Nematostella vectensis".Nature Ecology & Evolution 5.1(2021).