气候变化领域集成服务门户(CCPortal): Epidemiology of molecular probes in Xpert MTB/RIF assay in Khyber Pakhtunkhwa, Pakistan

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DOI	10.1007/s00203-021-02242-5
	Epidemiology of molecular probes in Xpert MTB/RIF assay in Khyber Pakhtunkhwa, Pakistan
	Khan, Anwar Sheed; Khan, Muhammad Tahir; Ali, Sajid; Khan, Taj Ali; Qasim, Muhammad; Malik, Arif; Sajjad, Wasim; ul ain, Qurrat; Irfan, Muhammad
通讯作者	Khan, MT (通讯作者)，Univ Lahore, Inst Mol Biol & Biotechnol, Lahore, Pakistan. ; Irfan, M (通讯作者)，Univ Florida, Coll Dent, Dept Oral Biol, Gainesville, FL 32610 USA.
发表日期	2021
ISSN	0302-8933
EISSN	1432-072X
起始页码	2249
结束页码	2256
卷号	203 期号:5
英文摘要	Regardless of a plethora of advanced diagnostics, TB and drug resistance remains a principal killer. We proposed gold nanoparticles (AuNPs) attached with probes to enhance the efficiency of GeneXpert MTB/RIF assay instead of conventional dye probes for molecular detection. A total of 15,000 samples were collected from TB suspects and subjected to Xpert MTB/RIF assay, where 6800 (45.3%) were detected as MTB positive, 280 (4.3%) were detected to harbor mutations in the RRDR, while invalid /errors were found in 690 (4.6%) cases. The mutations were detected by probe E, 199 (71.1%), while probes B and D, 30 and 26 (10% and 9%), respectively. In the Xpert MTB/RIF Assay were found mutations picked by probes E and B codons 529-533 (71%) and 512-518 (10%), respectively. The fast-rising works of TB nano-diagnostics, of Xpert probes, may improve by the applications of gold nanoparticle probes.
英文关键词	Xpert MTB; RIF assay; RRDR; AuNPs; Probe
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000622653100002
来源期刊	ARCHIVES OF MICROBIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/255071
作者单位	[Khan, Anwar Sheed; Ali, Sajid] Lab Hayatabad Med Complex, Prov TB Reference, Peshawar, Pakistan; [Khan, Muhammad Tahir; Malik, Arif] Univ Lahore, Inst Mol Biol & Biotechnol, Lahore, Pakistan; [Khan, Taj Ali; Qasim, Muhammad] Kohat Univ Sci & Technol, Dept Microbiol, Kohat, Pakistan; [Ali, Sajid] Abdul Wali Khan Univ, Dept Biotechnol, Mardan, Pakistan; [Sajjad, Wasim] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Lanzhou 7300000, Peoples R China; [ul ain, Qurrat] Quaid I Azam Univ, Dept Microbiol, Islamabad, Pakistan; [Irfan, Muhammad] Univ Florida, Coll Dent, Dept Oral Biol, Gainesville, FL 32610 USA
推荐引用方式 GB/T 7714	Khan, Anwar Sheed,Khan, Muhammad Tahir,Ali, Sajid,et al. Epidemiology of molecular probes in Xpert MTB/RIF assay in Khyber Pakhtunkhwa, Pakistan[J]. 中国科学院西北生态环境资源研究院,2021,203(5).
APA	Khan, Anwar Sheed.,Khan, Muhammad Tahir.,Ali, Sajid.,Khan, Taj Ali.,Qasim, Muhammad.,...&Irfan, Muhammad.(2021).Epidemiology of molecular probes in Xpert MTB/RIF assay in Khyber Pakhtunkhwa, Pakistan.ARCHIVES OF MICROBIOLOGY,203(5).
MLA	Khan, Anwar Sheed,et al."Epidemiology of molecular probes in Xpert MTB/RIF assay in Khyber Pakhtunkhwa, Pakistan".ARCHIVES OF MICROBIOLOGY 203.5(2021).