气候变化领域集成服务门户(CCPortal): Downregulation of miR156-Targeted PvSPL6 in Switchgrass Delays Flowering and Increases Biomass Yield

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DOI	10.3389/fpls.2022.834431
	Downregulation of miR156-Targeted PvSPL6 in Switchgrass Delays Flowering and Increases Biomass Yield
	Cai, Jinjun; Liu, Wenwen; Li, Weiqian; Zhao, Lijuan; Chen, Gang; Bai, Yangyang; Ma, Dongmei; Fu, Chunxiang; Wang, Yamei; Zhang, Xinchang
通讯作者	Zhang, XC (通讯作者)，Northwest Agr & Forestry Univ, Coll Nat Resources & Environm, Yangling, Peoples R China. ; Wang, YM (通讯作者)，Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China. ; Wang, YM (通讯作者)，Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS, Key Lab Biofuels, Qingdao, Peoples R China.
发表日期	2022
ISSN	1664-462X
卷号	13
英文摘要	MiR156/SQUAMOSA PROMOTER BINDING-LIKEs (SPLs) module is the key regulatory hub of juvenile-to-adult phase transition as a critical flowering regulator. In this study, a miR156-targeted PvSPL6 was identified and characterized in switchgrass (Panicum virgatum L.), a dual-purpose fodder and biofuel crop. Overexpression of PvSPL6 in switchgrass promoted flowering and reduced internode length, internode number, and plant height, whereas downregulation of PvSPL6 delayed flowering and increased internode length, internode number, and plant height. Protein subcellular localization analysis revealed that PvSPL6 localizes to both the plasma membrane and nucleus. We produced transgenic switchgrass plants that overexpressed a PvSPL6-GFP fusion gene, and callus were induced from inflorescences of selected PvSPL6-GFP(OE) transgenic lines. We found that the PvSPL6-GFP fusion protein accumulated mainly in the nucleus in callus and was present in both the plasma membrane and nucleus in regenerating callus. However, during subsequent development, the signal of the PvSPL6-GFP fusion protein was detected only in the nucleus in the roots and leaves of plantlets. In addition, PvSPL6 protein was rapidly transported from the nucleus to the plasma membrane after exogenous GA(3) application, and returned from the plasma membrane to nucleus after treated with the GA(3) inhibitor (paclobutrazol). Taken together, our results demonstrate that PvSPL6 is not only an important target that can be used to develop improved cultivars of forage and biofuel crops that show delayed flowering and high biomass yields, but also has the potential to regulate plant regeneration in response to GA(3).
关键词	BOUND TRANSCRIPTION FACTOR PANICUM VIRGATUM L.ARABIDOPSIS-THALIANA FLORAL INDUCTION MEMBRANE BIOLOGY STRESS-RESPONSE TIME BINDING PROTEIN TRANSFORMATION
英文关键词	PvSPL6; flowering; biomass yield; subcellular localization; gibberellin; switchgrass
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000766600700001
来源期刊	FRONTIERS IN PLANT SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/255032
作者单位	[Cai, Jinjun; Zhang, Xinchang] Northwest Agr & Forestry Univ, Coll Nat Resources & Environm, Yangling, Peoples R China; [Cai, Jinjun; Li, Weiqian; Chen, Gang; Bai, Yangyang] Ningxia Acad Agr & Forestry Sci, Inst Agr Resources & Environm, Yinchuan, Peoples R China; [Liu, Wenwen; Zhao, Lijuan; Fu, Chunxiang; Wang, Yamei] Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China; [Liu, Wenwen; Zhao, Lijuan; Fu, Chunxiang; Wang, Yamei] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS, Key Lab Biofuels, Qingdao, Peoples R China; [Zhao, Lijuan; Ma, Dongmei] Ningxia Univ, Breeding Base State Key Lab Land Degradat & Ecol, Yinchuan, Peoples R China; [Fu, Chunxiang] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
推荐引用方式 GB/T 7714	Cai, Jinjun,Liu, Wenwen,Li, Weiqian,et al. Downregulation of miR156-Targeted PvSPL6 in Switchgrass Delays Flowering and Increases Biomass Yield[J]. 中国科学院西北生态环境资源研究院,2022,13.
APA	Cai, Jinjun.,Liu, Wenwen.,Li, Weiqian.,Zhao, Lijuan.,Chen, Gang.,...&Zhang, Xinchang.(2022).Downregulation of miR156-Targeted PvSPL6 in Switchgrass Delays Flowering and Increases Biomass Yield.FRONTIERS IN PLANT SCIENCE,13.
MLA	Cai, Jinjun,et al."Downregulation of miR156-Targeted PvSPL6 in Switchgrass Delays Flowering and Increases Biomass Yield".FRONTIERS IN PLANT SCIENCE 13(2022).