气候变化领域集成服务门户(CCPortal): Lung transcriptome analysis for the identification of genes involved in the hypoxic adaptation of plateau pika (Ochotona curzoniae)

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DOI	10.1016/j.cbd.2021.100943
	Lung transcriptome analysis for the identification of genes involved in the hypoxic adaptation of plateau pika (Ochotona curzoniae)
	Zhang, Xu-Ze; Fu, Lin; Zou, Xiao-Yan; Li, Shuang; Ma, Xiao-Dong; Xie, Ling; Pang, Bo; Ma, Jian-Bin; Wang, Yu-Jun; Du, Yu-Rong; Guo, Song-Chang
通讯作者	Guo, SC (通讯作者)，Hunan Agr Univ, Sch Anim Sci & Technol, Changsha 410128, Peoples R China. ; Du, YR (通讯作者)，Qinghai Normal Univ, Key Lab Biodivers Format Mech, Xining 810008, Peoples R China.
发表日期	2022
ISSN	1744-117X
EISSN	1878-0407
卷号	41
英文摘要	The plateau pika, a typical hypoxia-tolerant mammal lives 3000-5000 m above sea level on the Qinghai-Tibet Plateau, has acquired many physiological and morphological characteristics and strategies in its adaptation to sustained, high-altitude hypoxia. Blunted hypoxic pulmonary vasoconstriction is one such strategy, but the genes involved in this strategy have not been elucidated. Here, we investigated the genes involved and their expression profiles in the lung transcriptome of plateau pikas subjected to different hypoxic conditions (using low-pressure oxygen cabins). A slight, right ventricular hypertrophy was observed in pikas of the control group (altitude: 3200 m) vs. those exposed to 5000 m altitude conditions for one week. Our assembly identified 67,774 genes; compared with their expression in the control animals, 866 and 8364 genes were co-upregulated and co-downregulated, respectively, in pikas subjected to 5000 m altitude conditions for 1 and 4 w. We elucidated pathways that were associated with pulmonary vascular arterial pressure, including vascular smooth muscle contraction, HIF-1 signalling, calcium signalling, cGMP-PKG signalling, and PI3K-Akt signalling based on the differentially expressed genes; the top-100 pathway enrichments were found between the control group and the group exposed to 5000 m altitude conditions for 4 w. The mRNA levels of 18 candidate gene showed that more than 83% of genes were expressed and the number of transcriptome The up-regulated genes were EPAS1, Hb alpha, iNOS, CX40, CD31, PPM1B, HIF-1 alpha, MYLK, Pcdh12, Surfactant protein B, the down-regulated genes were RYR2, vWF, RASA1, CLASRP, HIF-3 alpha. Our transcriptome data are a valuable resource for future genomic studies on plateau pika.
关键词	NITRIC-OXIDE SYNTHASE HIGH-ALTITUDE ADAPTATION PULMONARY VASOCONSTRICTION QINGHAI-TIBET ANGIOGENESIS INHIBITION HEMOGLOBIN VASORELAXATION HYPERTENSION CIRCULATION
英文关键词	Environmentally sensitive genes; Hypoxia adaptation; Plateau pika
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000776084600005
来源期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/255027
作者单位	[Zhang, Xu-Ze; Zou, Xiao-Yan; Guo, Song-Chang] Hunan Agr Univ, Sch Anim Sci & Technol, Changsha 410128, Peoples R China; [Zhang, Xu-Ze; Ma, Xiao-Dong] Qinghai Minzu Univ, Coll Ecol Environm & Resources, Xining 810007, Peoples R China; [Fu, Lin] Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China; [Zhang, Xu-Ze; Fu, Lin; Li, Shuang; Ma, Xiao-Dong; Wang, Yu-Jun] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Evolut & Adaptat Plateau Biota, Xining 810001, Peoples R China; [Xie, Ling] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China; [Pang, Bo] Hebei Univ Sci & Technol, Coll Food Sci & Biol, Shijiazhuang 050018, Hebei, Peoples R China; [Ma, Jian-Bin; Du, Yu-Rong] Qinghai Normal Univ, Key Lab Biodivers Format Mech, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xu-Ze,Fu, Lin,Zou, Xiao-Yan,et al. Lung transcriptome analysis for the identification of genes involved in the hypoxic adaptation of plateau pika (Ochotona curzoniae)[J]. 中国科学院西北生态环境资源研究院,2022,41.
APA	Zhang, Xu-Ze.,Fu, Lin.,Zou, Xiao-Yan.,Li, Shuang.,Ma, Xiao-Dong.,...&Guo, Song-Chang.(2022).Lung transcriptome analysis for the identification of genes involved in the hypoxic adaptation of plateau pika (Ochotona curzoniae).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,41.
MLA	Zhang, Xu-Ze,et al."Lung transcriptome analysis for the identification of genes involved in the hypoxic adaptation of plateau pika (Ochotona curzoniae)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 41(2022).