气候变化领域集成服务门户(CCPortal): Metagenomics reveals global-scale contrasts in nitrogen cycling and cyanobacterial light-harvesting mechanisms in glacier cryoconite

CCPortal

DOI	10.1186/s40168-022-01238-7
	Metagenomics reveals global-scale contrasts in nitrogen cycling and cyanobacterial light-harvesting mechanisms in glacier cryoconite
	Murakami, Takumi; Takeuchi, Nozomu; Mori, Hiroshi; Hirose, Yuu; Edwards, Arwyn; Irvine-Fynn, Tristram; Li, Zhongqin; Ishii, Satoshi; Segawa, Takahiro
通讯作者	Murakami, T (通讯作者)，Natl Inst Genet, Dept Informat, Shizuoka, Japan. ; Murakami, T (通讯作者)，Natl Inst Genet, Adv Genom Ctr, Shizuoka, Japan. ; Segawa, T (通讯作者)，Univ Yamanashi, Ctr Life Sci Res, Kofu, Yamanashi, Japan.
发表日期	2022
ISSN	2049-2618
卷号	10 期号:1
英文摘要	Background: Cryoconite granules are mineral-microbial aggregates found on glacier surfaces worldwide and are hotspots of biogeochemical reactions in glacier ecosystems. However, despite their importance within glacier ecosystems, the geographical diversity of taxonomic assemblages and metabolic potential of cryoconite communities around the globe remain unclear. In particular, the genomic content of cryoconite communities on Asia's high mountain glaciers, which represent a substantial portion of Earth's ice masses, has rarely been reported. Therefore, in this study, to elucidate the taxonomic and ecological diversities of cryoconite bacterial consortia on a global scale, we conducted shotgun metagenomic sequencing of cryoconite acquired from a range of geographical areas comprising Polar (Arctic and Antarctic) and Asian alpine regions. Results: Our metagenomic data indicate that compositions of both bacterial taxa and functional genes are particularly distinctive for Asian cryoconite. Read abundance of the genes responsible for denitrification was significantly more abundant in Asian cryoconite than the Polar cryoconite, implying that denitrification is more enhanced in Asian glaciers. The taxonomic composition of Cyanobacteria, the key primary producers in cryoconite communities, also differs between the Polar and Asian samples. Analyses on the metagenome-assembled genomes and fluorescence emission spectra reveal that Asian cryoconite is dominated by multiple cyanobacterial lineages possessing phycoerythrin, a green light-harvesting component for photosynthesis. In contrast, Polar cryoconite is dominated by a single cyanobacterial species Phormidesmis priestleyi that does not possess phycoerythrin. These findings suggest that the assemblage of cryoconite bacterial communities respond to regional- or glacier-specific physicochemical conditions, such as the availability of nutrients (e.g., nitrate and dissolved organic carbon) and light (i.e., incident shortwave radiation). Conclusions: Our genome-resolved metagenomics provides the first characterization of the taxonomic and metabolic diversities of cryoconite from contrasting geographical areas, highlighted by the distinct light-harvesting approaches of Cyanobacteria and nitrogen utilization between Polar and Asian cryoconite, and implies the existence of environmental controls on the assemblage of cryoconite communities. These findings deepen our understanding of the biodiversity and biogeochemical cycles of glacier ecosystems, which are susceptible to ongoing climate change and glacier decline, on a global scale.
关键词	EASTERN TIEN-SHAN NO. 1 NITRATE ASSIMILATION MICROBIAL ACTIVITY READ ALIGNMENT CARBON PHYCOBILISOME BACTERIAL QUALITY BIOGEOCHEMISTRY
英文关键词	Glacier ecosystem; Cryoconite; Metagenomics; Denitrification; Cyanobacteria; Phycobilisome; Chromatic acclimation
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000772080100001
来源期刊	MICROBIOME
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254988
作者单位	[Murakami, Takumi; Mori, Hiroshi] Natl Inst Genet, Dept Informat, Shizuoka, Japan; [Murakami, Takumi; Mori, Hiroshi] Natl Inst Genet, Adv Genom Ctr, Shizuoka, Japan; [Takeuchi, Nozomu] Chiba Univ, Grad Sch Sci, Dept Earth Sci, Chiba, Japan; [Hirose, Yuu] Toyohashi Univ Technol, Dept Appl Chem & Life Sci, Toyohashi, Aichi, Japan; [Edwards, Arwyn] Aberystwyth Univ, Inst Biol Environm & Rural Sci IBERS, Aberystwyth, Dyfed, Wales; [Edwards, Arwyn; Irvine-Fynn, Tristram] Aberystwyth Univ, Interdisciplinary Ctr Environm Microbiol, Aberystwyth, Dyfed, Wales; [Irvine-Fynn, Tristram] Aberystwyth Univ, Dept Geog & Earth Sci, Aberystwyth, Dyfed, Wales; [Li, Zhongqin] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Tien Shan Glaciol Stn, Lanzhou, Peoples R China; [Ishii, Satoshi] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA; [Ishii, Satoshi] Univ Minnesota, BioTechnol Inst, St Paul, MN 55108 USA; [Segawa, Takahiro] Univ Yamanashi, Ctr Life Sci Res, Kofu, Yama...
推荐引用方式 GB/T 7714	Murakami, Takumi,Takeuchi, Nozomu,Mori, Hiroshi,et al. Metagenomics reveals global-scale contrasts in nitrogen cycling and cyanobacterial light-harvesting mechanisms in glacier cryoconite[J]. 中国科学院西北生态环境资源研究院,2022,10(1).
APA	Murakami, Takumi.,Takeuchi, Nozomu.,Mori, Hiroshi.,Hirose, Yuu.,Edwards, Arwyn.,...&Segawa, Takahiro.(2022).Metagenomics reveals global-scale contrasts in nitrogen cycling and cyanobacterial light-harvesting mechanisms in glacier cryoconite.MICROBIOME,10(1).
MLA	Murakami, Takumi,et al."Metagenomics reveals global-scale contrasts in nitrogen cycling and cyanobacterial light-harvesting mechanisms in glacier cryoconite".MICROBIOME 10.1(2022).