气候变化领域集成服务门户(CCPortal): LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling

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DOI	10.1242/jcs.259468
	LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling
	Liu, Wenjing; Jiang, Xiaoyan; Li, Xiao; Sun, Kuanxiang; Yang, Yeming; Yang, Mu; Li, Shujin; Zhu, Xianjun
通讯作者	Yang, M ; Li, SJ ; Zhu, XJ (通讯作者)，Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Ctr Med Genet, Sch Med,Sichuan Prov Key Lab Human Dis Gene Study, Chengdu 610072, Sichuan, Peoples R China. ; Zhu, XJ (通讯作者)，Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China. ; Zhu, XJ (通讯作者)，Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China. ; Yang, M ; Li, SJ ; Zhu, XJ (通讯作者)，Sichuan Acad Med Sci, Res Unit Blindness Prevent, 2019RU026, Chengdu 610072, Sichuan, Peoples R China. ; Yang, M ; Li, SJ ; Zhu, XJ (通讯作者)，Sichuan Prov Peoples Hosp, Chengdu 610072, Sichuan, Peoples R China. ; Zhu, XJ (通讯作者)，First Peoples Hosp Shangqiu, Dept Ophthalmol, Shangqiu 476000, Henan, Peoples R China.
发表日期	2022
ISSN	0021-9533
EISSN	1477-9137
卷号	135 期号:6
英文摘要	Precise Norrin and beta-catenin (Norrin/beta-catenin; encoded by NDP and CTNNB1, respectively) signaling is critical for proper angiogenesis. Dysregulation of this signaling leads to various diseases, of which retinal exudative vitreoretinopathy is the most prevalent. Here, we used a global knockout mouse model to show that limb development membrane protein 1 like (LMBR1L), a transmembrane protein of unknown function in angiogenesis, is essential for retinal vascular development. In vitro experiments revealed that LMBR1L depletion results in aberrant activation of the Norrin/beta-catenin signaling pathway via decreased ubiquitylation of FZD4 and increased Norrin coreceptor LRP5 and p-GSK38-Ser9 expression levels, which cause accumulation of beta-catenin. Moreover, inhibition of LMBR1L in human retinal microvascular endothelial cells (HRECs) caused increased proliferation ability and defective cell migration, which might have occurred as a result of upregulated expression levels of the apical junction components. Treatment with p-GSK3 beta-Ser9 inhibitor AR-A014418 restored the phenotypes in LMBR1L null HRECs, which further demonstrated the important regulatory role of LMBR1L in the Norrin/beta-catenin signaling pathway. Taken together, our data reveal an essential role for LMBR1L in angiogenesis.
关键词	FAMILIAL EXUDATIVE VITREORETINOPATHY BETA-CATENIN RETINAL ANGIOGENESIS VASCULAR DEVELOPMENT WNT FRIZZLED-4 MUTATIONS LIPOCALIN-1 MECHANISMS EXPRESSION
英文关键词	beta-Catenin signaling; Angiogenesis; Knockout model; LMBR1L
语种	英语
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000779191300015
来源期刊	JOURNAL OF CELL SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254912
作者单位	[Liu, Wenjing; Jiang, Xiaoyan; Li, Xiao; Sun, Kuanxiang; Yang, Yeming; Yang, Mu; Li, Shujin; Zhu, Xianjun] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Ctr Med Genet, Sch Med,Sichuan Prov Key Lab Human Dis Gene Study, Chengdu 610072, Sichuan, Peoples R China; [Liu, Wenjing; Zhu, Xianjun] Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China; [Liu, Wenjing; Zhu, Xianjun] Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China; [Liu, Wenjing; Yang, Mu; Li, Shujin; Zhu, Xianjun] Sichuan Acad Med Sci, Res Unit Blindness Prevent, 2019RU026, Chengdu 610072, Sichuan, Peoples R China; [Liu, Wenjing; Yang, Mu; Li, Shujin; Zhu, Xianjun] Sichuan Prov Peoples Hosp, Chengdu 610072, Sichuan, Peoples R China; [Zhu, Xianjun] First Peoples Hosp Shangqiu, Dept Ophthalmol, Shangqiu 476000, Henan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenjing,Jiang, Xiaoyan,Li, Xiao,et al. LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling[J]. 中国科学院西北生态环境资源研究院,2022,135(6).
APA	Liu, Wenjing.,Jiang, Xiaoyan.,Li, Xiao.,Sun, Kuanxiang.,Yang, Yeming.,...&Zhu, Xianjun.(2022).LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling.JOURNAL OF CELL SCIENCE,135(6).
MLA	Liu, Wenjing,et al."LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/beta-catenin signaling".JOURNAL OF CELL SCIENCE 135.6(2022).