气候变化领域集成服务门户(CCPortal): Evidence for miRNAs involved in the high-altitude responses of sainfoin (Onobrychis viciifolia) grown in the Qinghai-Tibetan plateau

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DOI	10.1007/s13562-021-00702-z
	Evidence for miRNAs involved in the high-altitude responses of sainfoin (Onobrychis viciifolia) grown in the Qinghai-Tibetan plateau
	Yin, Hengxia; Zhou, Huakun; Wang, Wenying; Tran, Lam-Son Phan; Zhang, Benyin
通讯作者	Zhang, BY (通讯作者)，Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China. ; Tran, LSP (通讯作者)，Duy Tan Univ, Inst Res & Dev, 03 Quang Trung, Da Nang, Vietnam. ; Tran, LSP (通讯作者)，Texas Tech Univ, Dept Plant & Soil Sci, Inst Genom Crop Abiot Stress Tolerance, Lubbock, TX 79409 USA.
发表日期	2020
ISSN	0971-7811
EISSN	0974-1275
英文摘要	The identification of miRNAs and their functionality has mainly focused on the responses to single stresses or their combination under controlled conditions; however, the investigation of miRNAs under natural conditions is still limited. In the present study, miRNA-sequencing technology was used to identify differentially expressed miRNAs in the herbaceous plant, sainfoin (Onobrychis viciifolia), growing under the natural conditions present at low and high altitudes in the Qinghai-Tibetan plateau (QTP). In total, 298 known miRNAs and 190 novel putative miRNAs were identified, with miR319, miR166 and miR159 being the most highly represented miRNA families. In addition, results also revealed that 96 miRNAs, including 68 up-regulated and 28 down-regulated miRNAs, were differently expressed in sainfoin plants growing at a high-altitude, relative to those at a low altitude. Known miRNAs, such as miR156, miR160, miR397 and miR408, were among the up-regulated miRNAs, as well as novel-m0071 and novel-m0014. It is suggested that these miRNAs may play a crucial role in the adaption of sainfoin to the extreme natural environmental conditions present in the QTP. Additionally, a correlation analysis indicated that miR164 and miR5741 families had the largest number of target genes. As a result, they may play a primary role in the adaptation of sainfoin to adverse environmental conditions. The results obtained by stem-loop RT-qPCR analysis were consistent with the data obtained by high-throughput sequencing, confirming the accuracy of the miRNA sequencing. This is the first study to report on the identification of miRNAs in sainfoin under natural conditions, as well as differentially expressed miRNAs in sainfoin plants growing under natural contrasting environments, which will help elucidate the miRNA-mediated molecular mechanisms associated with the ability of sainfoin to adapt to the high-altitude environmental conditions in the QTP.
关键词	NAC TRANSCRIPTION FACTOR ABIOTIC STRESS MICRORNAS DROUGHT GENES IDENTIFICATION PERFORMANCE EXPRESSION RESISTANCE REVEALS
英文关键词	Extreme natural conditions; microRNAs; Qinghai-Tibetan plateau; Regulatory networks; Sainfoin
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000690801900001
来源期刊	JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254859
作者单位	[Yin, Hengxia; Zhang, Benyin] Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China; [Zhou, Huakun] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Restorat Ecol Cold Reg Qinghai Prov, Xining 810008, Peoples R China; [Wang, Wenying] Qinghai Normal Univ, Sch Life Sci, Xining 810008, Peoples R China; [Tran, Lam-Son Phan] Duy Tan Univ, Inst Res & Dev, 03 Quang Trung, Da Nang, Vietnam; [Tran, Lam-Son Phan] Texas Tech Univ, Dept Plant & Soil Sci, Inst Genom Crop Abiot Stress Tolerance, Lubbock, TX 79409 USA
推荐引用方式 GB/T 7714	Yin, Hengxia,Zhou, Huakun,Wang, Wenying,et al. Evidence for miRNAs involved in the high-altitude responses of sainfoin (Onobrychis viciifolia) grown in the Qinghai-Tibetan plateau[J]. 中国科学院西北生态环境资源研究院,2020.
APA	Yin, Hengxia,Zhou, Huakun,Wang, Wenying,Tran, Lam-Son Phan,&Zhang, Benyin.(2020).Evidence for miRNAs involved in the high-altitude responses of sainfoin (Onobrychis viciifolia) grown in the Qinghai-Tibetan plateau.JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY.
MLA	Yin, Hengxia,et al."Evidence for miRNAs involved in the high-altitude responses of sainfoin (Onobrychis viciifolia) grown in the Qinghai-Tibetan plateau".JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY (2020).