Climate Change Data Portal
| DOI | 10.3390/biology11030478 |
| An Alternative Class of Targets for microRNAs Containing CG Dinucleotide | |
| Dai, Wennan; Su, Xin; Zhang, Bin; Wu, Kejing; Zhao, Pengshan; Yan, Zheng | |
| 通讯作者 | Yan, Z (通讯作者)，Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Peoples R China. ; Yan, Z (通讯作者)，Lanzhou Univ, Coll Ecol, Lanzhou 730000, Peoples R China. ; Zhao, PS (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Stress Physiol & Ecol Cold & Arid Reg, Lanzhou 730000, Peoples R China. |
| 发表日期 | 2022 |
| EISSN | 2079-7737 |
| 卷号 | 11期号:3 |
| 英文摘要 | MicroRNAs (miRNAs) are endogenous similar to 23 nt RNAs which regulate message RNA (mRNA) targets mainly through perfect pairing with their seed region (positions 2-7). Several instances of UTR sequence with an additional nucleotide that might form a bulge within the pairing region, can also be recognized by miRNA as their target (bugle-target). But the prevalence of such imperfect base pairings in human and their roles in the evolution are incompletely understood. We found that human miRNAs with the CG dinucleotides (CG dimer) in their seed region have a significant low mutation rate than their putative binding sites in mRNA targets. Interspecific comparation shows that these miRNAs had very few conservative targets with the perfect seed-pairing, while potentially having a subclass of bulge-targets. Compared with the canonical target (perfect seed-pairing), these bulge-targets had a lower negative correlation with the miRNA expression, and either were down-regulated in the miRNA overexpression experiment or up-regulated in the miRNA knock-down experiment. Our results show that the bulge-targets are widespread in the miRNAs with CG dinucleotide within their seed regions, which could in part explain the rare conserved targets of these miRNAs based on seed rule. Incorporating these bulge-targets, together with conservation information, could more accurately predict the entire targets of these miRNAs. |
| 关键词 | CYTOSINE METHYLATIONRHO GTPASESRECOGNITIONPRINCIPLESPREDICTIONBIOGENESISEVOLUTIONSITESMODE |
| 英文关键词 | CG dinucleotide; miRNA; bulge; seed; non-canonical target |
| 语种 | 英语 |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| WOS类目 | Biology |
| WOS记录号 | WOS:000775843600001 |
| 来源期刊 | BIOLOGY-BASEL
 |
| 来源机构 | 中国科学院西北生态环境资源研究院 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/254829 |
| 作者单位 | [Dai, Wennan; Su, Xin; Yan, Zheng] Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Peoples R China; [Dai, Wennan; Su, Xin; Yan, Zheng] Lanzhou Univ, Coll Ecol, Lanzhou 730000, Peoples R China; [Zhang, Bin] King Abdullah Univ Sci & Technol KAUST, Computat Biosci Res Ctr CBRC, Comp Elect & Math Sci & Engn Div, Thuwal 239556900, Saudi Arabia; [Wu, Kejing] Chinese Acad Sci, Inst Pasteur Shanghai, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China; [Zhao, Pengshan] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Stress Physiol & Ecol Cold & Arid Reg, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Wennan,Su, Xin,Zhang, Bin,et al. An Alternative Class of Targets for microRNAs Containing CG Dinucleotide[J]. 中国科学院西北生态环境资源研究院,2022,11(3). |
| APA | Dai, Wennan,Su, Xin,Zhang, Bin,Wu, Kejing,Zhao, Pengshan,&Yan, Zheng.(2022).An Alternative Class of Targets for microRNAs Containing CG Dinucleotide.BIOLOGY-BASEL,11(3). |
| MLA | Dai, Wennan,et al."An Alternative Class of Targets for microRNAs Containing CG Dinucleotide".BIOLOGY-BASEL 11.3(2022). |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
