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DOI	10.1111/cmi.13391
	BSC2 induces multidrug resistance via contributing to the formation of biofilm in Saccharomyces cerevisiae
	Huang, Zhiwei; Dai, Hongsheng; Zhang, Xiaoyu; Wang, Qiao; Sun, Jing; Deng, Yunxia; Shi, Ping
通讯作者	Huang, ZW (通讯作者)，Donghua Univ, Coll Chem Chem Engn & Biotechnol, 2999 Renmin Rd, Shanghai 201620, Peoples R China.
发表日期	2021
ISSN	1462-5814
EISSN	1462-5822
卷号	23 期号:12
英文摘要	Biofilm plays an important role in fungal multidrug resistance (MDR). Our previous studies showed that BSC2 is involved in resistance to amphotericin B (AMB) through antioxidation in Saccharomyces cerevisiae. In this study, the overexpression of BSC2 and IRC23 induced strong MDR in S. cerevisiae. BSC2-overexpression affected cellular flocculation, cell surface hydrophobicity, biofilm formation and invasive growth. However, it failed to induce caspofungin (CAS) resistance and affect the invasive growth in FLO mutant strains (FLO11 Delta, FLO1 Delta, FLO8 Delta and TUP1 Delta). Furthermore, the overexpression of BSC2 compensated for chitin synthesis defects to maintain the cell wall integrity and significantly reduced the cell morphology abnormality induced by CAS. However, it could not repair the cell wall damage caused by CAS in the FLO mutant strains. Although BSC2 overexpression increased the level of mannose in the cell wall, DPM1 overexpression in both BY4741 and bsc2 increment could confer resistance to CAS and AMB. In addition, BSC2 overexpression significantly increased the mRNA expression of FLO11, FLO1, FLO8 and TUP1. BSC2 may function as a regulator of FLO genes and be involved in cell wall integrity in yeast. Taken together, our data demonstrate that BSC2 induces MDR in a FLO pathway-dependent manner via contributing to the formation of biofilms in S. cerevisiae. Take Aways Overexpression of BSC2 induced strong MDR in S. cerevisiae. BSC2 affected cellular flocculation, CSH, biofilm formation and invasive growth. BSC2 could not repair the cell wall damage caused by CAS in the FLO mutants. BSC2 may function as a regulator of FLO genes to maintain cell wall integrity. BSC2 promotes biofilm formation in a FLO pathway-dependent manner to induce MDR.
关键词	CELL-WALL DRUG-RESISTANCE CANDIDA-ALBICANS YEAST PROTEIN GENES GLYCOSYLATION MANNOSYLATION FLOCCULATION EXPRESSION
英文关键词	biofilm; BSC2; cell wall integrity; multidrug resistance; Saccharomyces cerevisiae
语种	英语
WOS研究方向	Cell Biology ; Microbiology
WOS类目	Cell Biology ; Microbiology
WOS记录号	WOS:000695132500001
来源期刊	CELLULAR MICROBIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254708
作者单位	[Huang, Zhiwei; Dai, Hongsheng; Zhang, Xiaoyu; Wang, Qiao; Deng, Yunxia] Donghua Univ, Coll Chem Chem Engn & Biotechnol, Key Lab Sci & Technol Ecotext, Minist Educ, Shanghai, Peoples R China; [Sun, Jing] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Qinghai Tibet Plateau Biol R, Xining, Peoples R China; [Shi, Ping] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Huang, Zhiwei,Dai, Hongsheng,Zhang, Xiaoyu,et al. BSC2 induces multidrug resistance via contributing to the formation of biofilm in Saccharomyces cerevisiae[J]. 中国科学院西北生态环境资源研究院,2021,23(12).
APA	Huang, Zhiwei.,Dai, Hongsheng.,Zhang, Xiaoyu.,Wang, Qiao.,Sun, Jing.,...&Shi, Ping.(2021).BSC2 induces multidrug resistance via contributing to the formation of biofilm in Saccharomyces cerevisiae.CELLULAR MICROBIOLOGY,23(12).
MLA	Huang, Zhiwei,et al."BSC2 induces multidrug resistance via contributing to the formation of biofilm in Saccharomyces cerevisiae".CELLULAR MICROBIOLOGY 23.12(2021).