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DOI	10.1016/j.cclet.2020.07.015
	Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters
	Liu, Lingyuan; Zhu, Shuyun; Sun, Jing; Xia, Meng; Zhao, Xian'en; Xu, Guobao
通讯作者	Zhu, SY ; Zhao, XE (通讯作者)，Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China.
发表日期	2021
ISSN	1001-8417
EISSN	1878-5964
起始页码	906
结束页码	909
卷号	32 期号:2
英文摘要	We design a ratiometric fluorescent sensing platform for bleomycin (BLM) by using proximity-dependent DNA-templated silver nanoclusters (DNA-AgNCs) probe. This ratiometric sensing system is constructed with DNA-AgNCs as single fluorophore. The proposed strategy is based on the two following facts: (1) a covert DNA can approach and transform the DNA-AgNCs with green emission (G-DNA-AgNCs) into red emission through hybridization reaction. (2) The specific cleavage of the convert DNA by BLM in the presence of Fe(II) inhibits the discoloration of G-DNA-AgNCs. Thus, benefiting from the specific recognition of BLM and unique properties of G-DNA-AgNCs, a highly-sensitive ratiometric sensor for BLM has been successfully developed. The detection limit is as low as 30 pmol/L. This label-free fluorescence probe possesses advantages of convenient synthetic process and low cost. Moreover, this ratiometric method has been applied to the detection of BLM in human serum samples, illustrating a promising tool for analysis of BLM in cancer therapy. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
英文关键词	Ratiometric nanosensor; DNA-templated silver nanoclusters; Fluorescence transformation; Bleomycin; DNA scission
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000635538300059
来源期刊	CHINESE CHEMICAL LETTERS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254641
作者单位	[Liu, Lingyuan; Zhu, Shuyun; Xia, Meng; Zhao, Xian'en] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China; [Sun, Jing] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Peoples R China; [Xu, Guobao] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lingyuan,Zhu, Shuyun,Sun, Jing,et al. Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters[J]. 中国科学院西北生态环境资源研究院,2021,32(2).
APA	Liu, Lingyuan,Zhu, Shuyun,Sun, Jing,Xia, Meng,Zhao, Xian'en,&Xu, Guobao.(2021).Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters.CHINESE CHEMICAL LETTERS,32(2).
MLA	Liu, Lingyuan,et al."Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters".CHINESE CHEMICAL LETTERS 32.2(2021).